Membrane Protein Purification and Crystallization
A Practical Guide
- Carola Hunte, Max-Planck-Institut fuer Biophysik, Frankfurt, Germany
- Gebhard von Jagow, Zentrum Für Biologische Chemie, Frankfurt, Germany
- Hermann Schagger, Zentrum Für Biologische Chemie, Universitätsklinikum Frankfurt, Germany
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.
Biochemists, molecular, cell, and structural biologists, and researchers in the pharmaceutical and biotechnical industry