Membrane Protein Purification and Crystallization

A Practical Guide

Edited by

  • Carola Hunte, Max-Planck-Institut fuer Biophysik, Frankfurt, Germany
  • Gebhard von Jagow, Zentrum Für Biologische Chemie, Frankfurt, Germany
  • Hermann Schagger, Zentrum Für Biologische Chemie, Universitätsklinikum Frankfurt, Germany

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.
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Biochemists, molecular, cell, and structural biologists, and researchers in the pharmaceutical and biotechnical industry


Book information

  • Published: December 2002
  • ISBN: 978-0-12-361776-7

Table of Contents

I. Strategies and TechniquesPurification Strategies for Membrane Proteins.Chromatographic Techniques and Basic Operations in Membrane Protein Purification.Production and purification of recombinant membrane proteins.SDS Electrophoresis Techniques.Blue-Native Electrophoresis. Preparative Isoelectric Focussing.Membrane protein crystallization. II. Discussion of Selected Isolation ProtocolsLipid Dependent Inactivation and Reactivation of Bovine complex III.Purification of affinity-epitope tagged G-protein coupled receptors.Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D Crystallization.Purification of the yeast cytochrome bc1 complex. III. Crystallization of Membrane ProteinsAntibody fragment-mediated crystallization of membrane proteins.Crystallization of Wolinella succinogenes quinol:fumarate reductase.Ba3-type cytochrome-c oxidase from Thermus thermophilus:purification, crystallization, and crystal transformation.Two-dimensional crystallization of membrane proteins: a practical guide.In cubo crystallization of membrane proteins.