Table of ContentsPart A. Bacteria 1. Where are we in the exploration of Escherichia coli translocationpathways? (B. Wickner). 2. Components involved in bacterial proteintranslocation (C. Harris, P.C. Tai). 3. Molecular characterization of Sec proteinscomprising the protein secretory machinery of Escherichia coli (S. Mizushima,H. Tokuda, S. Matsuyama). 4. Distinct steps in the insertion pathway ofbacteriophage coat proteins (A. Kuhn, D. Troschel). 5. Steps in the assembly of acytoplasmic membrane protein - The MalF component of the maltose transportcomplex (B. Traxler, J. Beckwith). 6. Structural characteristics of presecretoryproteins: Their implication as to translocation competency (S. Mizushima, K. Tani,C. Hikita, M. Kato). 7. Sequence determinants of membrane protein topology(G. von Heijne). 8. Lipid involvement in protein translocation (B. de Kruijff,E. Breukink, R.A. Demel, R. van 't Hof, H.H.J. de Jongh, W. Jordi, R.C.A. Keller, J.A. Killian, A.I.P.M. de Kroon, R. Kusters,M. Pilon). Part B. Endoplasmic reticulum 9. Membrane protein insertion into theendoplasmic reticulum - signals, machinery, and mechanisms (S. High,B. Dobberstein). 10. Translocation of proteins through the endoplasmic reticulum membrane: investigation of their molecular environment by crosslinking (E. Hartmann,T.A. Rapoport). 11. The role of GTP in protein targeting to the endoplasmicreticulum (C. Ogg, J.M. Nunnari, J.D. Miller, P. Walter). 12. Consecutive steps of nucleosidetriphosphate hydrolysis are driving transport of precursor proteins into theendoplasmic reticulum (P. Klappa, G. Müller, G. Schlenstedt, H. Wiech, R. Zimmermann).Part C. Vacuoles 13. Mechanisms and regulation of import and degradation ofcytosolic proteins in the lysosome/vacuole (H.-L. Chiang, R. Schekman). 14. The sortingof soluble and integral membrane proteins to the yeast vacuole (C.K. Raymond,C.A. Vater, S. Nothwehr, C.J. Roberts, T.H. Stevens). Part D. Peroxisomes 15. Definingcomponents required for peroxisome assembly in Saccharomyces cerevisiae(J. Höhfeld, D. Mertens, F.F. Wiebel, W.-H. Kunau). 16. Structure and assembly ofperoxisomal membrane proteins (J.M. Goodman, L.J. Garrard, M.T. McCammon). 17. Mechanisms oftransport of proteins into microbodies (S. Subramani). 18. Lessons for peroxisomebiogenesis from fluorescence analyses of Zellweger syndrome fibroblasts(P.B. Lazarow, H.W. Moser, M.J. Santos). Part E. Mitochondria 19. The mitochondrial proteinimport machinery of Saccharomyces cerevisiae (V. Hines). 20. Protein insertioninto mitochondrial outer and inner membranes via the stop-transfer sortingpathway (G.C. Shore, D.G. Millar, J.-M. Li). 21. General and exceptional pathways of proteinimport into sub-mitochondrial compartments (R. Lill, C. Hergersberg, H. Schneider,T. Söllner, R. Stuart, W. Neupert). Part F. Cloroplasts 22. Targeting ofproteins into and across the chloroplast envelope (H.-M. Li, S.E. Perry, K. Keegstra). 23.Transport of proteins into the thylakoids of higher plant chloroplasts (C. Robinson).24. Comparison of two different protein translocation mechanisms intochloroplasts (J. Soll, H. Alefsen, B. Böckler, B. Kerber, M. Salomon, K. Waegemann). Part G.Chaperones 25. DnaJ homologs and protein transport (T. Kurihara, P.A. Silver). 26.Chaperonin-mediated protein folding (A.L. Horwich, S. Caplan, J.S. Wall, F.-U.Hartl). References. Index.