Measurement and Manipulation of Intracellular Ions

Edited by

  • Jacob Kracier, The University of Western Ontario, Health Sciences Centre, London, Canada and Human Frontier Science Program, Strasbourg, France
  • S. Dixon, The University of Western Ontario, Health Sciences Centre, London, Canada


  • P. Michael Conn, Texas Tech University Health Sciences Center, Lubbock, USA

This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures.
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Book information

  • Published: September 1995
  • ISBN: 978-0-12-185297-9

Table of Contents

Light-Optical-Based Reagents for the Measurement and Manipulation of Ions, Metabolites, and Macromolecules in Living Cells, K.A. Giuliano and D.L. Taylor.Use of Ion-Selective Microelectrodes for Recording Intracellular Ion Levels, M.E. Morris.Use of NMR Spectroscopy to Measure Intracellular Ion Concentrations, S.J. Karlik.Use of Ionophores for Manipulating Intracellular Ion Concentrations, G.A. Woolley, D.R. Pfeiffer, and C.M. Deber.Fluorescence Imaging of Cytosolic Calcium: An Introduction to Basic Experimental Principles, D.A. Williams.Fluorescence Measurements of Cytosolic Calcium: Combined Photometry with Electrophysiology, W.T. Mason, J. Dempster, R. Zorec, J. Joyland, and P.M. Lledo.Use of Chelators and Photoactivatable Caged Compounds to Manipulate Cytosolic Calcium, A.M. Gurney and S.E. Bates.Measurement of Cytosolic Calcium by 19 NMR, G.A. Smith and H.S. Bachelard.Fluorescence Measurement of Cytosolic pH in Cultured Rodent Astrocytes, S.J. Dixon and J.X. Wilson.Brain Cell Acid-Base Changes Measured with Ion-Selective Microelectrodes, C.D. Lascola and R.P. Kraig.Measurement of Cytosolic pH by Nuclear Magnetic Resonance Spectroscopy, O.A.C. Petroff and J.W. Prichard.Manipulation and Regulation of Cytosolic pH, M.O. Bevensee and W.F. Boron.Fluorescence Measurements of Cytosolic Sodium Concentration, M.A. Robertson and J.K. Foskett.Measurement of Cytosolic Sodium Using Ion-Selective Microelectrodes, J.W. Deitmer and T. Munsch.A Practical Guide to the Use of Fluorescent Indicators for the Measurement of Cytosolic Free Magnesium, E. Murphy and R.E. London.Measurement of Cytosolic Magnesium by 31P NMR Spectroscopy, J.A. Helpern and H.R. Halvorson.Chloride-Sensitive Fluorescent Indicators, A.S. Verkman and J. Biwersi.Measurement of Cytosolic Chloride Activity by Ion-Selective Microelectrodes, J.A. Coles.Use of Ion-Selective Microelectrodes and Fluorescent Probes to Measure Cell Volume, F.J. Alvarez-Leefmans, J. Altamirano, and W.E. Crowe.Index.