Manual of Techniques in Invertebrate Pathology
- 2nd Edition - April 25, 2012
- Editor: Lawrence A. Lacey
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 3 8 6 8 9 9 - 2
- eBook ISBN:9 7 8 - 0 - 1 2 - 3 8 6 9 0 0 - 5
Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay,… Read more
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Request a sales quote- Step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens
- New edition fully updated to address changes in the taxonomy of the vast majority of taxa
- Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques
- Provides extensive supplemental literature and recipes for media, fixatives and stains
Dedication
Contributors
Preface to first edition
Preface to second edition
Chapter I. Initial handling and diagnosis of diseased invertebrates
1 Introduction
2 Collection of diseased invertebrates and entomopathogens
3 Examination in the laboratory
4 Safety considerations
Acknowledgments
REFERENCES
Chapter II. Basic techniques in insect virology
1 Introduction
2 Brief overview of the different groups of insect viruses
3 Identification
4 Virus propagation
5 Virus quantification
6 Quantification of virus activity (virulence)
7 Cell culture techniques
8 Reverse genetics
REFERENCES
Glossary
Appendix: Recipes for stains
Chapter III. Isolation, culture, preservation, and identification of entomopathogenic bacteria of the Bacilli
1 Introduction
2 Isolation
3 Cultivation
4 Preservation
5 Identification
6 Concluding remarks
Acknowledgments
Disclaimer
REFERENCES
Appendix
Chapter IV. Bioassay of bacterial entomopathogens against insect larvae
1 Introduction
2 Laboratory bioassay of Bacillus pathogens against lepidopteran larvae
3 Bioassay of Bacillus thuringiensis against dipteran larvae
4 Bioassay of Bacillus thuringiensis against other orders
5 Bioassay of other entomopathogens
6 Conclusions
Acknowledgments
REFERENCES
Appendix: artificial diet recipes
Chapter V. Bacteria for use against soil-inhabiting insects
1 Introduction
2 Isolation
3 Identification
4 Propagation and production
5 Bioassay
6 Preservation
REFERENCES
Appendix 1
Chapter VI. Identification of entomopathogenic fungi
1 Introduction
2 Quick guide to light microscopy
3 Key to major genera of fungal entomopathogens
4 Major genera of fungal entomopathogens
REFERENCES
Notes
Brief glossary of mycological terms
Appendix: recipes of stains and reagents
Chapter VII. Laboratory techniques used for entomopathogenic fungi: Hypocreales
1 Introduction
2 Detection and characterization
3 Inoculum propagation and preparation
4 Quantification
5 Infection and bioassay
6 Assessment of environmental parameters
Acknowledgments
REFERENCES
Appendix
Chapter VIII. Mass production of entomopathogenic Hypocreales
1 Introduction
2 Culture maintenance
3 Solid Substrate Production of Aerial Conidia
4 Liquid Fermentation Production
Acknowledgments
REFERENCES
Appendix
Chapter IX. Methods for study of the Entomophthorales
1 Introduction
2 Collecting and preparing specimens
3 Species identification
4 Isolation
5 In vitro culture and production
6 In vivo culture and production
7 Bioassays
Acknowledgments
REFERENCES
Appendix: media
Chapter X. Preservation of entomopathogenic fungal cultures
1 Introduction
2 Preservation at temperatures above freezing
3 Preservation by freeze-drying (lyophilization)
4 Preservation in a frozen state
5 Record keeping for storage facilities
6 Other less common preservation techniques
REFERENCES
Chapter XI. Research methods for entomopathogenic microsporidia and other protists
1 Introduction
2 Identification
3 Techniques for managing spores
4 Molecular and proteomic techniques
5 Propagation and production
6 Controlling infections in insect colonies
7 Bioassays and experimental infections
Acknowledgments
REFERENCES
Chapter XII. Nematode parasites, pathogens and associates of insects and invertebrates of economic importance
1 Introduction
2 Major groups of nematodes associated with insects and other invertebrates of economic importance
3 Sampling techniques
4 Diagnostic methods
5 Staining methods
6 Culturing methods
7 Nematode quantification methods
8 Preservation methods
9 Shipping and handling of samples
10 Bioassays with entomopathogenic nematodes and symbiotic bacteria
Acknowledgments
REFERENCES
Appendix I
Appendix II
Chapter XIII. Pathogens and parasites of terrestrial molluscs
1 Introduction
2 General techniques for working with terrestrial mollucs
3 Major groups of pathogens and parasites associated with terrestrial molluscs
Acknowledgments
REFERENCES
Appendix
Chapter XIV. Testing the pathogenicity and infectivity of entomopathogens to mammals
1 Introduction
2 Definition of infection
3 Definition of persistence
4 Mammalian safety tests and philosophy
5 Animal procurement and housing
6 Modified tier scheme
7 Conclusion
Acknowledgments
REFERENCES
Chapter XV. Complementary techniques: preparations of entomopathogens and diseased specimens for more detailed study using microscopy
1 Introduction
2 Light microscopy
3 Electron microscopy
B Scanning electron microscopy (SEM)
Disclaimer
Acknowledgments
REFERENCES
Appendix
Index
Color Plates
- No. of pages: 504
- Language: English
- Edition: 2
- Published: April 25, 2012
- Imprint: Academic Press
- Paperback ISBN: 9780123868992
- eBook ISBN: 9780123869005
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