Low-Abundance Proteome Discovery

State of the Art and Protocols


  • Egisto Boschetti, Scientific Consultant, Paris, France
  • Pier Giorgio Righetti, Miles Gloriosus Academy, Milano, Italy

Low-Abundance Proteome Discovery addresses the most critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. The book describes an original strategy developed by the authors that permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries. Known as combinatorial peptide ligand libraries, these new methodologies are one of the hottest topics related to the study of proteomics and have applications in medical diagnostics, food quality, and plant analysis. The book is written for university and industry scientists starting proteomic studies of complex matrices (e.g., biological fluids, biopsies, recalcitrant plant tissues, foodstuff, and beverage analysis), researchers doing wet chemistry, and graduate-level students in the areas of analytical and biochemistry, biology, and genetics.
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Analytical chemists, biochemists, clinical chemists, biologists, including graduate-level students in these disciplines; genetic and biomedical researchers


Book information

  • Published: May 2013
  • Imprint: ELSEVIER
  • ISBN: 978-0-12-401734-4


"Boschetti and Righetti introduce the new technique of combinatorial peptide ligand libraries for identifying low-abundance peptide fragments. The standard proteomics techniques of mass spectrometry, 2D electrophoresis and chromatography are deliberately given brief treatment only as prefractionation tools in favor of new methodologies."--Reference & Research Book News, October 2013

Table of Contents

1. Introducing low-abundance species in proteome analysis
2. Chromatographic and electrophoretic pre-fractionation tools in proteome analysis
3. Current low-abundance protein access
4. Low-abundance protein access by combinatorial peptide libraries
5. Plant proteomics and food and beverage analysis via CPLL capture
6. Biomedical involvements of low-abundance proteins
7. Other applications of combinatorial peptide libraries
8. Detailed methodologies and protocols