Lipids book cover

Lipids

Lipids are a broad group of naturally occurring molecules which includes fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides, phospholipids, and others. The main biological functions of lipids include energy storage, as structural components of cell membranes, and as important signaling molecules.
This volume of Methods in Cell Biology covers such areas as Membrane structure and dynamics, Imaging, and Lipid Protein Interactions. It will be an essential tool for researchers and students alike.

Audience

Researchers and students in cell, molecular and developmental biology

Included in series
Methods in Cell Biology

Hardbound, 528 Pages

Published: February 2012

Imprint: Academic Press

ISBN: 978-0-12-386487-1

Contents

  • Membrane Dynamics and Reconstitution Assays:

    1. Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding
    2. Min Wu and Pietro De Camilli

    3. Studying lipids involved in the endosomal pathway
    4. Christin Bissig, Shem Johnson, and Jean Gruenberg

    5. Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking
    6. Jean-Baptise Manneville, Cécile Leduc, Benoit Sorre, and Guillaume Drin

    7. Reconstituting Multivesicular Body Biogenesis with purified components
    8. Thomas Wollert

    9. Approaches to the study of Atg8-mediated membrane dynamics in vitro
    10. Anjali Jotwani, Diana N. Richerson, Isabelle Motta, Omar Julca-Zevallos, and Thomas J. Melia

    11. Reconstitution assay system for ceramide transport with semi-intact cells
    12. Keigo Kumagai, Masahiro Nishijim, Kentaro Hanada

    13. Visualizing mitochondrial lipids and fusion events in mammalian cells
    14. Huiyan Huang and Michael A. Frohman

      Lipid Metabolism and Signaling:

    15. Targeted and non-targeted analysis of membrane lipids using mass spectrometry
    16. Xue Li Guan and Markus R. Wenk

    17. Modulation of host phosphoinositide metabolism during Salmonella invasion by the type III secreted effector SopB
    18. Bernhard Roppenser, Sergio Grinstein and John H. Brumell

    19. Acute Manipulation of Phosphoinositide Levels in Cells
    20. Belle Chang-Ileto, Samuel G. Frere, and Gilbert Di Paolo

    21. Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins
    22. Marnix Wieffer, Volker Haucke and Michael Krauß

    23. Phosphoinositides at the neuromuscular junction of Drosophila melanogaster: a genetic approach.
    24. Jan R. Slabbaert Thang Manh Khuong and Patrik Verstreken

    25. Devising powerful Genetics, Biochemical and Structural tools in the Functional Analysis of Phosphatidylinositol Transfer Proteins (PITPs) across diverse species
    26. Jim Davison, Vytas A. Bankaitis and Ratna Ghosh

    27. Genome-wide screens for gene products regulating lipid droplet dynamics.
    28. Weihua Fei and Hongyuan Yang

      Imaging:

    29. The Three Dimensionality of Cell Membranes: Lamellar to Cubic Membrane Transition as Investigated by Electron Microscopy
    30. Ketpin Chong and Yuru Deng

    31. Quantitative imaging of lipid metabolism in yeast: from 4D analysis to high content screens of mutant libraries
    32. Heimo Wolinski, Kristian Bredies and Sepp D. Kohlwein

    33. Analysis of cholesterol trafficking with fluorescent probes
    34. Frederick R. Maxfield and Daniel Wüstner

    35. Fluorescence Correlation Methods for Imaging Cellular Behavior of Sphingolipid-Interacting Probes
    36. Rachel Kraut, Nirmalya Bag, and Thorsten Wohland

    37. Monitoring Phospholipid Dynamics during Phagocytosis: Application of Genetically-Encoded Fluorescent Probes
    38. Helen Sarantis and Sergio Grinstein

    39. Genetically encoded probes for phosphatidic acid

              Nawal Kassas, Petra Tryoen-Tóth, Matthias Corrotte, Tamou Thahouly, Marie-France Bader, Nancy J.Grant and Nicolas Vitale

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