Light and Video Microscopy book cover

Light and Video Microscopy

The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation.

cell biologists, neurobiologists, reproductive biologists, plant biologists, microbiologists, fungal biologists, veterinarians, forensic scientists, food scientists, horticulturalists, biochemists, physicists, engineers, and anyone using a light or video microscope

Hardbound, 366 Pages

Published: January 2014

Imprint: Academic Press

ISBN: 978-0-12-411484-5


  • "As such, this book would be an excellent undergraduate-level text on optical microscopy for biologists. It is also valuable to anyone using a light microscope."--Carol Bayles, Cornell University, Ithaca, NY, USA


  • Chapter 1: The Relation Between the Object and the Image
    Chapter 2: Geometrical Optics
    Chapter 3: Physical Optics
    Chapter 4: Bright Field Microscopy
    Chapter 5: Phase Contrast and Dark Field Microscopy, Rheinberg and Oblique Illumination
    Chapter 6: Photomicrography
    Chapter 7: Fluorescence Microscopy
    Chapter 8: Polarization Microscopy
    Chapter 9: Interference Microscopy
    Chapter 10: Differential Interference Microscopy
    Chapter 11: Modulation Contrast Microscopy
    Chapter 12: Video and Digital Microscopy
    Chapter 13: Image Processing and Analysis
    Chapter 14: Various Kinds of Microscopes and Accessories


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