Laser Capture in Microscopy and Microdissection book cover

Laser Capture in Microscopy and Microdissection

Methods in Enzymology

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences.

Audience
Biochemists, cell and molecular biologists, geneticists, biomedical researchers, and microbiologists.

Included in series
Methods in Enzymology

Hardbound, 775 Pages

Published: October 2002

Imprint: Academic Press

ISBN: 978-0-12-182259-0

Reviews

  • "The Methods in Enzymology series represents the gold-standard."
    PRAISE FOR THE SERIES, —NEUROSCIENCE


    "Incomparably useful."
    —ANALYTICAL BIOCHEMISTRY
    "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
    —BIO/TECHNOLOGY
    "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
    —CHEMISTRY IN INDUSTRY
    "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
    —AMERICAN SOCIETY OF MICROBIOLOGY NEWS
    "If we had some way to find the work most often consulted in the laboratory, it could well be Colowick and Kaplan's multi-volume series Methods in Enzymology...a great work."
    —ENZYMOLOGIA
    "A series that has established itself as a definitive reference for biochemists."
    —JOURNAL OF CHROMATOGRAPHY

Contents

  • Comparison of Current Equipment. Laser Capture Microdissection (LCMD) and its Applications in Genomics & Proteomics. Going in vivo With Laser MD (LMD). Use of LCMD to Selectively Obtain Distinct Populations of Cells for Proteomic Analysis. Optimized Tissue Processing and Staining for LCMD and Nucleic Acid Retrieval. Fluoroscence in situ Hybridization of LCM-isolated Nuclei from Paraffin Sections. Immunoblotting of Single Cell Types Isolated from Frozen Sections by LMD. Noncontact Laser Catapulting: A Basic Procedure for Functl' Genomics and Proteomics. Internal Standards for LMD. Methacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded Tissues. Use of LCMD for Clonal Analysis. LCMD in Carcinoma Analysis. LCMD to Assess Devt. Application of LCMD to Proteomics. LCMD of the Mouse Intestine: Characterizing mRNA and Protein Expression, and Profiling Intermediary Metabolism in Specified.LCMD in Pathology. Use of Laser Capture Microscopy (LCM) in the Analysis of Mouse Models of Human Diseases. Use of LMD in Complex Tissue. Assessmt of Clonal Relationships in Malignant Melanomas. Comp. of Normal and Tumor Cells by LCMD. Analysis of Folliculostellate Cells by LCMD and Reverse Transcription-Polymerase Chain Reaction (LCM-RT/PCR). Analysis of Gene Expression. Analysis of Specific Gene Expression. Gene Discovery With LCM. DNA Fingerprinting from Cells Captured by LMD. Single Cell PCR in LCM.Assessmt of Genetic Clonality in Tumors using LCMD. Gene Mutations: Analysis in Proliferative Prostatic Diseases Using LCMD. Use of LCMD Generated Targets for Hybridization of High-Density Oligonucleotide Arrays. Single Cell Gene Mutation Analysis Using Laser-Assisted MD of Tissue Sections. Methylation in Gene Promoters: Assessmt after LCMD.

Advertisement

advert image