Landmark Papers in Clinical Chemistry

Edited by

  • Richard M. Rocco, Department of Biology, San Francisco State University, California, USA

This is the first major review of the developments in clinical laboratory science in the 20th century presented in the words of the original inventors and discoverers. Introductory comments by the editor help place the works within the historical context.Landmark Papers addresses:*The origin of the home pregnancy test available today in every drugstore*The woman who invented a billion dollar technology, refused to patent it and went on to win a Nobel Prize*The scientists who worked on the US Government’s crash program at the start of WWII to find a substitute for the malaria drug quinine*The blood test used to monitor the effectiveness of cholesterol lowering drugs that today are taken by over 20 million patients*The graduate student who invented a technology for testing for infectious diseases, took it to Africa to screen people for malaria for the first time and which is now used to test for HIV infection world-wide*The invention of molecular diagnostics by Linus Pauling and the road to individualized medicine*The development of the glucose meter used by diabetics up to six times a day to monitor their metabolic control
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Students and workers in the clinical laboratory field; Teachers in the clinical laboratory field; Clinical Chemists; Clinical Pathologists; Medical Laboratory Technologists; Industries that have commercialized the technologies (i.e., Abbott, Beckman, Pharmacia, etc...)


Book information

  • Published: November 2005
  • Imprint: ELSEVIER
  • ISBN: 978-0-444-51950-4


"With these landmark papers, an interested reader can easily trace the origins of the techniques that are being used in clinical laboratories today and the analytes being measured with them." --Carl A. Burtis, Chief Clinical Chemistry, Health Division at Oak Ridge National Laboratory, TN "...highlights the extensive and varied scientific basis of the discipline we call clinical chemistry. Of particular importance, it rekindles memories of the giants who have placed this discipline for taking care of people in our hands." --Amadeo J. Pesce, Director of Clinical Toxicology Laboratory, University of Cincinnati Hospital, OH

Table of Contents

PrefaceSection I IMMUNOASSAY TECHNOLOGYRIA (Radioimmunoassay) 1. Yalow, R.S., Berson, S.A. (1960: Assay of plasma insulin in man.FPIA (Fluorescence Polarization Immunoassay) 2. Dandliker, W.B., Feigen, G.A. (1961): Quantification of the antigen-antibody reaction by the polarization of fluorescence. CPB (Competitive Protein Binding) 3. Murphy, B.E.P., Pattee, C.J. (1964): Determination of thyroxine utilizing the property of protein-binding. ELISA (Enzyme-Linked Immunosorbent Assay) 4. Engvall, E., Perlmann, P. (1972): Enzyme-linked immunosorbent assay, ELISA. III.EMITä (Enzyme Immunoassay Technique) 5. Rubenstein, K.E., Schneider, R. S., Ullman, E.F. (1972): "Homogeneous" enzyme immunoassay. Section II THERAPEUTIC DRUG MONITORING (TDM)Bromide 6. Wuth, O. (1927): Rational bromide treatment. Sulfonamides 7. Bratton, A.C., Marshall, E.K., Jr., (1939): A New Coupling Component for Sulfanilamide Determination. Quinine and Quinidine 8. Brodie, B.B., Udenfriend, S. (1943): The estimation of quinine in human plasma with a note on the estimation of quinidine. Digoxin 9. Smith, T.W., Butler, V.P., Haber, E. (1969): Determination of therapeutic and toxic serum digoxin concentrations by radioimmunoassay. Theophylline 10. Thompson, R.D., Nagasawa, H.T., Jenne, J.W. (1974): Determination of theophylline and its metabolites in human urine and serum by high-pressure liquid chromatography. Section III ENZYMOLOGYALP (Alkaline Phosphatase, EC 11. Bessey, O.A., Lowry, O.H., Brock, M.J. (1946): A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum.AST (Aspartate Aminotransferase, EC 12. Karmen, A., Wroblewski, F., LaDue, J.S. (1955): Transaminase activity in human blood. CK (Creatine Kinase, EC 13. Mercer, D.W. (1974): Separation of tissue and serum creatine kinase isoenzymes by ion-exchange column chromatography. Section IV SPECIFIC ANALYTES Colorimetric Analysis with the Duboscq 14. Folin, O., Wu, H. (1919): A system of blood analysis. Blood Gases by Manometer 15. Van Slyke, D.D. (1924): The determination of gases in blood and other solutions by vacuum extraction and manometric measurement. I. Bilirubin, Total and Direct With the Filter Photometer 16. Malloy, H.T., Evelyn, K.A. (1937): The determination of bilirubin with the photometric colorimeter.Proteins by the Biuret Reaction 17. Gornall, A. G., Bardawill, C. J., David, M. M. (1949): Determination of serum proteins by means of the Biuret reaction.Methods for Pediatric and Neonatal Samples 18. Natelson, S. (1951): Routine use of ultramicro methods in the clinical laboratory.Enzymatic Glucose 19. Huggett, A.S., Nixon, D.A. (1957): Use of glucose oxidase, peroxidase, and o-dianisidine in determination of blood and urinary glucose. HCG (Human Chorionic Gonadotropin) by Immunoassay 20. Wide, L., Gemzell, C.A. (1960): An immunological pregnancy test.Hemoglobin A1c 21. Rahbar, S. (1968): An Abnormal Hemoglobin in Red Cells of Diabetics. LDL (Low Density Lipoprotein) Cholesterol by Calculation 22. Friedewald, W.T., Levy, R.I., Fredrickson, D.S. (1972): Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge.Section V INSTRUMENTATION AND TECHNIQUESPaper Electrophoresis 23. Durrum, E.L. (1950): A microelectrophoretic and microionophoretic technique. Automated Analyzer 24. Skeggs, L.T. (1957): An automatic method for colorimetric analysis. Enzymatic Glucose, Dry Reagents on Pads 25. Free, A.H., Adams, E.C., Kercher, M.L., Free, H. M., Cook, M. H., (1957): Simple Specific Test for Urine Glucose.Blood Gas Elelctrodes 26. Severinghaus, J., Bradley, A.F. (1958): Electrodes for blood pO2 and pCO2 determination. Specific Ion Electrodes27. Friedman, S.M., Nakashima, M. (1961): Single sample analysis with the sodium electrode.Centrifugal Analyzer 28. Anderson, N.G. (1969): Analytical techniques for cell fractions. XII. A multiple-cuvet rotor for a new microanalytical system. Immunoassays on Membranes 29. Glad, C., Grubb, A.O. (1978): Immunocapillary migration- A new method for immunochemical quantitation. Western Blots 30. Towbin, H., Stachelin, T., Gordon, J. (1979): Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications.Capillary Electrophoresis (CE) 31. Jorgenson, J.W., Lukacs, K. DeA. (1981): Zone electrophoresis in open-tubular glass capillaries. Section VI CHEMOMETRICSQuality Control Charts 32. Levey, S., Jennings, E.R. (1950): The Use of Control Charts in the Clinical Laboratory.Interferences in Analytical Accuracy 33. Caraway, W. (1962): Chemical and Diagnostic Specificity of Laboratory Tests.Predictive Value Theory 34. Vecchio, T.J. (1966): Predictive value of a single diagnostic test in unselected populations. Standards and Calibrators 35. Radin, N. (1967): What is a standard?Test Kits 36. Barnett, R.N., Cash, A.D., Junghams, S.P. (1968): Performance of "kits" used for clinical chemical analysis of cholesterol. Section VII MOLECULAR DIAGNOSTICSBiochemical Genetics 37. Pauling, L., Itano, H.A., Singer, S.J., Wells., I.C. (1949): Sickle cell anemia, a molecular disease. Nucleic Acid Probes 38. Hyypia, T., Jalava, A., Larsen, S.H., Terho, P., Hukkanene, V. (1985): Detection of Chlamydia trachomatis in clinical specimens by nucleic acid spot hybridization. Microarrays 39. Maskos, U., Southern, E.M. (1992): Oligonucleotide hybridizations on glass supports: A novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotide synthesized in situ. AcknowledgmentsAuthor IndexSubject Index