Laboratory Methods in Enzymology: RNA book cover

Laboratory Methods in Enzymology: RNA

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 472 Pages

Published: September 2013

Imprint: Academic Press

ISBN: 978-0-12-420037-1


  • Computational Prediction of RNA Secondary Structure

    Reverse-transcription PCR (RT-PCR)

    Northern blotting

    Explanatory Chapter: Nuclease Protection Assays

    In Vitro Transcription from Plasmid or PCR-amplified DNA

    Measurement of In Vivo RNA Synthesis Rates

    Method for measuring mRNA decay rate in Saccharomyces cerevisiae

    Analysis of polysomes from bacteria

    Polysome Profile Analysis – Yeast

    Polysome analysis of mammalian cells

    Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae

    Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates

    Circularized RT-PCR (cRT-PCR): analysis of the 5′ ends, 3′; ends, and poly(A) tails of RNA

    RNA Radiolabeling

    Fluorescently Labeling Synthetic RNAs

    Analysis of RNA by analytical polyacrylamide gel electrophoresis

    RNA purification by preparative polyacrylamide gel electrophoresis

    Explanatory Chapter: Nucleic Acid Concentration Determination

    RNA purification – precipitation methods

    Reverse Transcriptase Dideoxy Sequencing of RNA

    RNA Structure Experimental Analysis – Chemical Modification

    Structural Analysis of RNA Backbone Using In-Line Probing


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