Laboratory Methods in Enzymology: Protein Part B book cover

Laboratory Methods in Enzymology: Protein Part B

Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts.

Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 240 Pages

Published: March 2014

Imprint: Academic Press

ISBN: 978-0-12-420120-0

Contents

  • Small-scale Expression of Proteins in E. coli

    Protein Expression-Yeast

    Recombinant protein expression in baculovirus-infected insect cells

    Single Cell Cloning of a Stable Mammalian Cell Line

    In vitro synthesis of proteins in bacterial extracts

    Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation

    Yeast Two-Hybrid Screen

    UV cross-linking of interacting RNA and protein in cultured cells

    Analysis of RNA–protein interactions by cell mixing

    General protein–protein cross-linking

    Chromatin Immunoprecipitation and Multiplex Sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans

    PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins

    Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system

    Dissecting a known RNA–protein interaction using a yeast three-hybrid system

    Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system

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