Laboratory Methods in Enzymology: Protein Part B

Edited by

  • Jon Lorsch, Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA

Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts.
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Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

 

Book information

  • Published: March 2014
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-420120-0


Table of Contents

Small-scale Expression of Proteins in E. coli

Protein Expression-Yeast

Recombinant protein expression in baculovirus-infected insect cells

Single Cell Cloning of a Stable Mammalian Cell Line

In vitro synthesis of proteins in bacterial extracts

Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation

Yeast Two-Hybrid Screen

UV cross-linking of interacting RNA and protein in cultured cells

Analysis of RNA–protein interactions by cell mixing

General protein–protein cross-linking

Chromatin Immunoprecipitation and Multiplex Sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans

PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins

Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system

Dissecting a known RNA–protein interaction using a yeast three-hybrid system

Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system