Laboratory Methods in Enzymology: Protein Part A book cover

Laboratory Methods in Enzymology: Protein Part A

The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few.

This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including:

  • Pulse-chase analysis to measure protein degradation
  • Labeling a protein with fluorophores using NHS ester derivitization
  • Immunoaffinity purification of proteins
  • Proteolytic affinity tag cleavage
  • Purification of GST-tagged proteins

Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 200 Pages

Published: January 2014

Imprint: Academic Press

ISBN: 978-0-12-420070-8

Contents

  • Practical Steady-state Enzyme Kinetics
    Quantification of Protein Concentration using UV absorbance and Coomassie Dyes
    Preparation of protein samples for mass spectrometry and N-terminal sequencing
    Lysis of mammalian and Sf9 cells
    In Vivo [35 S]-Methionine Incorporation
    Pulse-chase analysis to measure protein degradation
    Labeling of a Protein with Fluorophores using Maleimide Derivitization
    Labeling a protein with fluorophores using NHS ester derivitization
    Protein Derivitization-Expressed Protein Ligation
    Protein Biotinylation
    Purification of His-tagged proteins
    Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag
    Immunoaffinity purification of proteins
    Affinity Purification of Protein Complexes Using TAP Tags
    Strep-tagged protein purification
    Proteolytic affinity tag cleavage
    Affinity Pull-down of Proteins using anti-FLAG M2 Agarose Beads
    Protein Affinity Purification using Intein/Chitin Binding Protein Tags
    Purification of GST-tagged proteins

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