Contributors to Volume 294.Preface.Volumes in Series.Physical Methods:Optical Measurements of Calcium Signals in Mammalian Presynaptic Terminals, P. Saggau, R. Gray and J.A. Dani.Fluorescent Techniques for Measuring Ion Channel Activity, G. Velicelebi, K.A. Strauderman, M.A. Varney, M. Akong, S.D. Hess and E.C. Johnson.Tagging Potassium Ion Channels with Green Fluorescent Protein to Study Mobility and Interactions with Other Proteins, E.S. Levitan.Spin Label Electron Spin Resonance and Fourier Transform Spectroscopy for Structural/Dynamic Measurements on Ion Channels, D. Marsh.Nuclear Magnetic Resonance Spectroscopy of Peptide Ion Channel Ligands: Cloning and Expression as Aid to Evaluation of Structural and Dynamic Properties, M.D. Reily, A.M. Bokman, J. Offord and P. McConnell.Ligand Binding Methods for Analysis of Ion Channel Structure and Function, S.E. Pedersen, M.M. Lurtz and R.V.L. Papineni.Three-Dimensional Structure of Membrane Proteins Determined by Two-Dimensional Crystallization, Electron Cryomicroscopy, and Image Analysis, M. Yeager, V.M. Unger and A.K. Mitra.Drug-Dependent Ion Channel Gating by Application of Concentration Jumps Using U-Tube Technique, F. Bretschneider and F. Markwardt.Voltage Clamp Biosensors for Capillary Electrophoresis, O. Orwar, K. Jardemark, C. Farre, I. Jacobson, A. Moscho, J.B. Shear, H.A. Fishman, S.J. Lillard and R.N. Zare.Ion Channels as Tools to Monitor Lipid Bilayer-Membrane Protein Interactions: Gramicidin Channels as Molecular Force Transducers, O.S. Andersen, C. Nielsen, A.M. Maer, J.A. Lundbaek, M. Goulian and R.E. Koeppe, III.Purification and Reconstitution:Purification and Reconstitution of Epithelial Chloride Channel Cystic Fibrosis Transmembrane Conductance Regulator, M. Ramjeesingh, E. Garami, L.-J. Huan, K. Galley, C. Li, Y. Wang and C.E. Bear.Purification, Characterization, and Reconstitution of Cyclic Nucleotide-Gated Channels, R.S. Molday and L.L. Molday.Purification and Heterologous Expression of Inhibitory Glycine Receptors, B. Laube and H. Betz.Purification and Functional Reconstitution of High-Conductance Calcium-Activated Potassium Channel from Smooth Muscle, M.L. Garcia, K.M. Giangiacomo, M. Hanner, H.-G. Knaus, O.B. McManus, W.A. Schmalhofer and G.J. Kaczorowski.Reconstitution of Native and Cloned Channels into Planar Bilayers, I. Favre, Y.-M. Sun and E. Moczydlowski.Iodide Channel of the Thyroid: Reconstituting Ion Channels into Planar Lipid Bilayers, P.E. Golstein, A. Sener, F. Colin and R. Beauwens.Nystatin/Ergosterol Method for Reconstituting Ion Channels into Planar Lipid Bilayers, D.J. Woodbury.Isolation of Transport Vesicles That Deliver Ion Channels to the Cell Surface, S. Sattsangi and W.F. Wonderlin.Second Messengers and Biochemical Approaches:Protein Phosphorylation of Ligand-Gated Ion Channels, A.L. Mammen, S. Kamboj and R.L. Huganir.Analysis of Ion Channel Associated Proteins, M. Wyszynski and M. Sheng.Signal Transduction through Ion Channels Associated with Excitatory Amino Acid Receptors, K. Ogita and Y. Yoneda.Secondary Messenger Regulation of Ion Channels/Plant Patch Clamping, S.M. Assmann and L. Romano.Special Channels:ATP-Sensitive Potassium Channels, M. Schwanstecher, C. Schwanstecher, F. Chudziak, U. Panten, J.P. Clement IV, G. Gonzalez, L. Aguilar-Bryan and J. Bryan.Mechanosensitive Channels of Bacteria, P. Blount, S.I. Sukharev, P.C. Moe, B. Martinac and C. Kung.Simplified Fast Pressure-Clamp Technique for Studying Mechanically Gated Channels, D.W. McBride, Jr. and O.P. Hamill.Virus Ion Channels, D.C. Ogden, I.V. Chizhmakov, F.M. Geraghty and A.J. Hay.Ion Channels in Microbes, Y. Saimi, S.H. Loukin, X.-L. Zhou, B. Martinac and C. Kung.Design and Characterization of Gramicidin Channels, D.V. Greathouse, R.E. Koeppe II, L.L. Providence, S. Shobana and O.S. Andersen.Functional Analyses of Aquaporin Water Channel Proteins, P. Agre, J.C. Mathai, B.L. Smith and G.M. Preston.Toxins and Other Membrane Active Compounds:Pore-Blocking Toxins as Probes of Voltage-Dependent Channels, R.J. French and S.C. Dudley, Jr..Conus Peptides as probes for Ion Channels, J.M. McIntosh, B.M. Olivera and L.J. Cruz.Scorpion Toxins as Tools for Studying Potassium Channels, M.L. Garcia, M. Hanner, H.-G. Knaus, R. Slaughter and G.J. Kaczorowski.Potassium Ion Channel Inactivation Peptides, R.D. Murrell-Lagnado.Interactions of Snake Dendrotoxins with Potassium Channels, W.F. Hopkins, M. Allen and B.L. Tempel.Use of Planar Lipid Bilayer Membranes for Rapid Screening of Membrane Active Compounds, T.A. Mirzabekov, A.Y. Silberstein and B.L. Kagan.Reagent and Information Sources:Antibodies to Ion Channels, A.C. Vincent, I.K. Hart, A. Pinto and F.A. Stephenson.Internet Information on Ion Channels: Issues of Access and Organization, E.C. Conley.Author Index.Subject Index.