Introduction to Electron Microscopy for Biologists

Methods in Cell Biology

Edited by

  • Terry Allen, Ph.D.,D.Sc., Paterson Institute, University of Manchester, UK

This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers).
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Researchers in all fields of biology who use electron microscopy in their lab and studies.


Book information

  • Published: July 2008
  • ISBN: 978-0-12-374320-6

Table of Contents

PART ONE. Exploring the Organisation of the Cell by Electron MicroscopyBasic Transmission and Scanning Electron Microscopy1. Scanning Electron Microscopy at the sub-cellular level-Terry Allen2 Electron Probe X-ray microanalysis for the study of cell physiology.E. Fernandez-Segura and A.Warley3 Preparation of cells and tissues for Immuno EM Paul Webster, Heinz Schwarz and Gareth Griffiths4 Fixation, Freezing and Embedding for Genetically Altered TissueHeinz Schwarz5 Combined video fluorescence and 3-D electron microscopyAlexander A. Mironov, Roman S. Polishchuk and Galina V. Beznoussenko 6. From Live-cell Imaging to Scanning Electron Microscopy (SEM): The Use of Green Fluorescent Protein (GFP) as a Common LabelSheona P Drummond and Terry D Allen 7 Immunolabelling for High Resolution Scanning Electron Microscopy .- Martin Goldberg 8 Immunogoldlabeling of Thawed CryosectionsPeter J. Peters and Jason Pierson 9. Close-to-Native Ultrastructural Preservation by High Pressure FreezingDimitri Vanhecke, Werner Graber, and Daniel Studer10. High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire CoverslipsSiegfried Reipert and Gerhard Wiche 11 Freeze-fracture Cytochemistry in Cell BiologyNicholas J. Severs and Horst Robenek PART TWO. Electron Microscopy of Specific Cellular StructureThe Cell Membrane12 The Plasma membrane cytoskeleton Akihiro KusumiThe Cytoskeleton13. Correlated Light and Electron Microscopy of the CytoskeletonSonja Auinger and J.Victor Small14 Electron microscopy of intermediate filaments: Teaming up with atomic force and confocal laser scanning microscopyLaurent Kreplak, Karsten Richter, Ueli Aebi and Harald Herrmann 15. Studying Microtubules by Electron MicroscopyCarolyn Moores Cytoplasm, Extracellular Matrix and Cell Junctions16. Endoplasmic reticulum and Golgi Complex. John Lucoqc17. Visualization of Dynamins Jason A. Mears and Jenny E. Hinshaw 18. Electron Microscopy of Collagen Fibril Structure In Vitro And In Vivo including Three-Dimensional ReconstructionTobias Starborg, Yinhui Lu, Karl E. Kadler and David F. Holmes19 Visualisation of Desmosomes in the Electron MicroscopeAnthea Scothern & David Garrod The Nucleus20 A Protocol for Isolation and Visualization of Yeast Nuclei by Scanning Electron Microscopy (SEM)Stephen Murray and Elena Kiseleva21. High Pressure Freezing and Freeze Substitution of S.pombe and S.cerevisiae for TEMStephen Murray22. Electron Microscopy of Lamin and the Nuclear Lamina in Caenorhabditis elegansMerav Cohen, Rachel Santarella, Naama Wiesel, Iain Mattaj, and Yosef Gruenbaum23. Visualization of Nuclear Organisation by Ultrastructural CytochemistryMarco Biggiogera and Stanislav Fakan 24. Scanning Electron Microscopy of ChromosomesGerhard Wanner and Elizabeth Schroeder-Reiter PART THREE Cells and Infectious Agents25. Infection at the Cellular LevelChristian Goosmann, Ulrike Abu Abed, and Volker Brinkmann26. Electron Microscopy of Viruses and Virus-Cell InteractionsPeter Wild