In Situ Molecular Pathology and Co-Expression AnalysesEdited by
- Gerard Nuovo, Ohio State University Medical Center, Columbus, OH, USA
This new laboratory reference manual will explain, in easy-to-understand language, simplified ways to understand and perform in situ hybridization and immunohistochemistry tests. The book will also focus much attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses.
The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols and regimens for troubleshooting to maximize the use of these platforms.
Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry, especially with computer based co-expression analyses, offer a large advantage to researchers competing for federal moneys or private grants.
Pathologists, clinical chemists, and toxicologists; practicing clinicians, fellows, and residents in pathology
Hardbound, 298 Pages
Published: March 2013
Imprint: Academic Press
3. The Biochemical Basis of In Situ Hybridization and Immunohistochemistry
4. The Basics of In Situ Hybridization
5. The Basics of Immunohistochemistry
6. The Basics of Histologic Interpretations of Tissues
7. The Recommended Protocol for In Situ Hybridization
8. The Recommended Protocol for Immunohistochemistry
9. Co-expression Analyses
10. PCR In Situ Hybridization and RT In Situ PCR