Immunology of Infection

Edited by

  • Stefan Kaufmann, Max Planck Institute for Infection Biology, Berlin, Germany
  • Dieter Kabelitz, University of Kiel, Germany

Immunology of Infection, 2nd Edition, edited by two leading experts in the field, presents the most appropriate up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the Microbiologist interested in analysing host-pathogen relationships, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology and parasitology.This new edition of Immunology of Infection provides ready-to-use "recipes", and the latest emerging techniques as well as novel approaches to the tried and tested, established methods included in the successful first edition.Methods in Microbiologyis the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.
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Microbiologists, immunologists, mycologists, and parasitologists


Book information

  • Published: November 2002
  • ISBN: 978-0-12-521532-9


Praise for the first edition: "This is a useful compendium of methods and techniques for those wishing to examine the immune responses to infections in vitro and in vivo." "The book is clearly presented, packed with protocols, and well illustrated" —Microbiology Today " represents a valuable addition to the microbiologists' reference source" —Immunology Today Praise for the Series: "An invaluable resource for the detailed analysis of cellular gene function and genome architecture." —NATURE

Table of Contents

ContributorsPreface to the 2nd editionPreface to the 1st editionIntroductionThe immune response to infectious agentsGeneral MethodsPhenotyping and separation of leukocyte populations based on affinity labellingCytometric cytokine secretion assay: detection and isolation of cytokine secreting T cellsMeasurement of cellular proliferationUse of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope driven vaccine designThe use of tetramers in the quantitative analysis of T cell responsesUsing microarrays for studying the host transcriptional response to microbial infectionGenome-wide expression profiling of intracelllular bacteria: the interaction of Mycobacterium tuberculosis with macrophagesMurine ModelsManagement of immuno-compromised and infected animalsIn Vitro AnalysisIsolation and preparation of lymphocytes from infected animals for in vitro analysisIsolation and development of murine T-lymphocyte hybridomas and clonal cell linesKiller cell assaysQuantitation of T Cell Cytokine Responses by ELISA, ELISPOT, Flow Cytometry and Reverse Transcriptase-PCR MethodsIsolation of and measuring the function of professional phagocytes: murine macrophagesIn vivo AnalysisThe immune response in mice challenged with bacterial infectionsMeasuring immune responses in vivoSpecific ModelsMurine model of tuberculosisThe leishmaniasis modelAnimal models: murine cytomegalovirusDNA vaccines: fundamentals and practicePreparation and use of adjuvantsHuman SystemsIsolation and propagation of human dendritic cellsIsolation of T cells and establishment of T-cell lines and clonesGrowth-transformation of human T cellsGeneration and characterisation of human killer cellsMeasuring human cytokine responsesMeasuring immune responses in situ: immunofluorescent and immunoenzymatic techniquesIsolation, characterisation and cultivation ofhuman monocytes and macrophages