Immunology of Infection

Edited by

  • Stefan Kaufmann, Max Planck Institute for Infection Biology, Berlin, Germany
  • Dieter Kabelitz, University of Kiel, Germany

Immunology of Infection, 2nd Edition, edited by two leading experts in the field, presents the most appropriate up-to-date experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the Microbiologist interested in analysing host-pathogen relationships, this volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology and parasitology.This new edition of Immunology of Infection provides ready-to-use "recipes", and the latest emerging techniques as well as novel approaches to the tried and tested, established methods included in the successful first edition.Methods in Microbiologyis the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.
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Microbiologists, immunologists, mycologists, and parasitologists


Book information

  • Published: December 2002
  • ISBN: 978-0-12-402033-7


"This is a very useful book that should not be kept in a bookshelf but will certainly be very helpful if put on the laboratory bench." -INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY (2003) "The book is clearly presented, packed with protocols, and well illustrated" —MICROBIOLOGY TODAY " represents a valuable addition to the microbiologists' reference source" —IMMUNOLOGY TODAY Praise for the Series: "An invaluable resource for the detailed analysis of cellular gene function and genome architecture." —NATURE

Table of Contents

ContributorsPreface to the 2nd editionPreface to the 1st editionIntroductionThe immune response to infectious agentsGeneral MethodsPhenotyping and separation of leukocyte populations based on affinity labelling.Cytometric cytokine secretion assay: detection and isolation of cytokine secreting T cells.Measurement of cellular proliferation.Use of bioinformatics to predict MHC ligands and T-cell epitopes: application to epitope driven vaccine design.The use of tetramers in the quantitative analysis of T cell responses.Using microarrays for studying the host transcriptional response to microbial infection.Genome-wide expression profiling of intracelllular bacteria: the interaction of Mycobacterium tuberculosis with macrophages.Murine ModelsManagement of immuno-compromised and infected animals.In Vitro AnalysisIsolation and preparation of lymphocytes from infected animals for in vitro analysis.Isolation and development of murine T-lymphocyte hybridomas and clonal cell lines.Killer cell assays.Quantitation of T Cell Cytokine Responses by ELISA, ELISPOT, Flow Cytometry and Reverse Transcriptase-PCR Methods.Isolation of and measuring the function of professional phagocytes: murine macrophages.In vivo AnalysisThe immune response in mice challenged with bacterial infections.Measuring immune responses in vivo.Specific ModelsMurine model of tuberculosis.The leishmaniasis model.Animal models: murine cytomegalovirus. DNA vaccines: fundamentals and practice.Preparation and use of adjuvants.Human SystemsIsolation and propagation of human dendritic cells.Isolation of T cells and establishment of T-cell lines and clones.Growth-transformation of human T cells.Generation and characterisation of human killer cells.Measuring human cytokine responses.Measuring immune responses in situ: immunofluorescent and immunoenzymatic techniques.Isolation, characterisation and cultivation of human monocytes and macrophages.