Edited by

  • P. Michael Conn, Texas Tech University Health Sciences Center, Lubbock, USA

This volume of Methods in Enzymology is the first of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.
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Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists


Book information

  • Published: January 2012
  • ISBN: 978-0-12-391857-4

Table of Contents

Part A: Techniques

  1. Laser Induced Radiation Microbeam Technology and Simultaneous Real-time Fluorescence Imaging in Live Cells
  2. Stanley W. Botchway, Pamela Reynolds, Anthony W. Parker, Peter O’Neill

  3. A cell biologist’s guide to high resolution imaging
  4. Graeme Ball, Richard M Parton, Russell S Hamilton and Ilan Davis

  5. Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo
  6. André E.P. Bastos, Silvia Scolari, Martin Stöckl, Rodrigo F.M. de Almeida

  7. Detecting and Tracking Non-Fluorescent Nanoparticle Probes in Live Cells
  8. Gufeng Wang and Ning Fang

  9. Fluorescence lifetime microscopy of tumor cell invasion, drug delivery and cytotoxicity
  10. Gert-Jan Bakker, Volker Andresen, Robert M. Hoffman, Peter Friedl

  11. Measuring membrane protein dynamics in neurons using Fluorescence recovery after photobleach (FRAP)
  12. Inmaculada M González-González, Frédéric Jaskolski, Yves Goldberg, Michael C. Ashby and Jeremy M. Henley

  13. Distinctive characteristics of Madeira wine regarding its traditional winemaking and modern analytical methodologies
  14. Rosa Perestrelo, Francisco Albuquerque, Sílvia M. Rocha, José S. Câmara

  15. Green to red photo-convertible mEosFP aided live imaging in plants
  16. Jaideep Mathur, Sarah Griffiths, Kiah Barton and Martin H. Schattat

  17. Methods for Cell and Particle Tracking
  18. Erik Meijering, Oleh Dzyubachyk, Ihor Smal

  19. Correlative light-electron microscopy: a potent tool for the imaging of rare or unique cellular and tissue events and structures
  20. Alexander A. Mironov and Galina V. Beznoussenko

  21. Optical Techniques for Imaging Membrane Domains in Live Cells (Live Cell Palm of Protein Clustering)
  22. Dylan M. Owen, David Williamson, Astrid Magenau, and Katharina Gaus

  23. Single live cell topography and activity imaging with the Shear-Force-Based constant-distance scanning electrochemical microscope
  24. Albert Schulte, Michaela Nebel and Wolfgang Schuhmann

  25. Visualization of TGN-endosome trafficking in mammalian and drosophila cells
  26. Satoshi Kametaka and Satoshi Waguri

  27. Live cell imaging with chemical specificity using dual frequency CARS microscopy.
  28. Iestyn Pope, Wolfgang Langbein, Paola Borri and Peter Watson

  29. Imaging intracellular protein dynamics by spinning disk confocal microscopy
  30. Samantha Stehbens, Hayley Pemble, Lindsay Murrow and Torsten Wittmann

    Part B: Tools

  31. Visualizing Dynamic Activities of Signaling Enzymes using genetically encodable FRET-based biosensors: from designs to applications
  32. Xin Zhou, Katie J. Herbst, Jin Zhang

  33. Live-cell Imaging of Aquaporin-4 Supramolecular Assembly and Diffusion
  34. A.S. Verkman, Andrea Rossi and Jonathan M. Crane

  35. Coiled-coil tag–probe labeling methods for live-cell imaging of membrane receptors
  36. Yoshiaki Yano, Kenichi Kawano, Kaoru Omae, and Katsumi Matsuzaki

  37. Monitoring Protein Interactions in Living Cells with Fluorescence Lifetime Imaging Microscopy
  38. Yuansheng Sun, Nicole M. Hays, Ammasi Periasamy, Michael W. Davidson, and Richard N. Day

  39. Open Source Tools for Fluorescent Imaging
  40. Nicholas A. Hamilton

  41. Nanoparticle PEBBLE sensors in live cells

    Yong-Eun Koo Lee and Raoul Kopelman