IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS

IMAGING LIVE CELLS IN HEALTH AND DISEASE

Edited by

  • P. Michael Conn, Texas Tech University Health Sciences Center, Lubbock, USA

This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.
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Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists

 

Book information

  • Published: February 2012
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-391856-7


Table of Contents

A: Viruses, Microbes and Parasites

Nanoscale imaging of microbial pathogens using atomic force microscopy

David

Alsteens

Challenges in imaging parasites

Friedrich

Frischknecht

Real-time live imaging to study bacterial infections in vivo

Agneta

Richter-Dahlfors

Analysis of cellular membrane dynamics by following virus endocytosis

Mario

Schelhaas

Imaging of live malaria blood stage parasites

Tobias

Spielmann

Escherichia coli K1 invasion of human brain microvascular endothelial cells

Theresa H.

Ward

B: Disease States

Real-time imaging after cerebral ischemia

Jasna

Kriz

Imaging acute inflammation in zebrafish

Graham J.

Lieschke

Imaging protein oligomerization in neurodegeneration using BiEC

Tiago Fleming

Outeiro

Imagng and quantifying the dyanmics of tumor-associated proteolysis

Bonnie F.

Sloane

C: Other Techniques

Live cell imaging in vivo with fluorescent proteins

Robert M.

Hoffman

Visualization of gene expression

Banafshe

Larijani

Imaging stem cell implant for cellular-based therapies

Zhenglong

Lee

Live cell single molecule imaging to investigate early events in B lymphocyte signaling

Susan K.

Pierce

A quantitative method for measuring phototoxicity of a live cell imaging microscope

Spencer L.

Shorte

High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines

Davide

Danovi

High-content screening of primary neurons

Steven

Finkbeiner

Live-imaging fluorescent proteins in early mouse embryos

Anna Katerina

Hadjantonakis

Comparing the methods for quantitative analysis of dendritic spine dynamics

Pirta

Hotulainen

Live-imaging of cell competition in drosophila imaginal epithelium

Tatsushi

Igaki

Live cell imaging of the oviduct

Sabine

Kölle

Computational estimation of leukocyte numbers in zebrafish

Graham J.

Lieschke

Four dimensional tracking of lymphocyte migration and interactions by two-photon microscopy

Taka

Okada