This volume of Methods in Enzymology is the first of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists

Included in series
Methods in Enzymology

Hardbound, 560 Pages

Published: January 2012

Imprint: Academic Press

ISBN: 978-0-12-391857-4


  • Part A: Techniques

    1. Laser Induced Radiation Microbeam Technology and Simultaneous Real-time Fluorescence Imaging in Live Cells
    2. Stanley W. Botchway, Pamela Reynolds, Anthony W. Parker, Peter O’Neill

    3. A cell biologist’s guide to high resolution imaging
    4. Graeme Ball, Richard M Parton, Russell S Hamilton and Ilan Davis

    5. Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo
    6. André E.P. Bastos, Silvia Scolari, Martin Stöckl, Rodrigo F.M. de Almeida

    7. Detecting and Tracking Non-Fluorescent Nanoparticle Probes in Live Cells
    8. Gufeng Wang and Ning Fang

    9. Fluorescence lifetime microscopy of tumor cell invasion, drug delivery and cytotoxicity
    10. Gert-Jan Bakker, Volker Andresen, Robert M. Hoffman, Peter Friedl

    11. Measuring membrane protein dynamics in neurons using Fluorescence recovery after photobleach (FRAP)
    12. Inmaculada M González-González, Frédéric Jaskolski, Yves Goldberg, Michael C. Ashby and Jeremy M. Henley

    13. Distinctive characteristics of Madeira wine regarding its traditional winemaking and modern analytical methodologies
    14. Rosa Perestrelo, Francisco Albuquerque, Sílvia M. Rocha, José S. Câmara

    15. Green to red photo-convertible mEosFP aided live imaging in plants
    16. Jaideep Mathur, Sarah Griffiths, Kiah Barton and Martin H. Schattat

    17. Methods for Cell and Particle Tracking
    18. Erik Meijering, Oleh Dzyubachyk, Ihor Smal

    19. Correlative light-electron microscopy: a potent tool for the imaging of rare or unique cellular and tissue events and structures
    20. Alexander A. Mironov and Galina V. Beznoussenko

    21. Optical Techniques for Imaging Membrane Domains in Live Cells (Live Cell Palm of Protein Clustering)
    22. Dylan M. Owen, David Williamson, Astrid Magenau, and Katharina Gaus

    23. Single live cell topography and activity imaging with the Shear-Force-Based constant-distance scanning electrochemical microscope
    24. Albert Schulte, Michaela Nebel and Wolfgang Schuhmann

    25. Visualization of TGN-endosome trafficking in mammalian and drosophila cells
    26. Satoshi Kametaka and Satoshi Waguri

    27. Live cell imaging with chemical specificity using dual frequency CARS microscopy.
    28. Iestyn Pope, Wolfgang Langbein, Paola Borri and Peter Watson

    29. Imaging intracellular protein dynamics by spinning disk confocal microscopy
    30. Samantha Stehbens, Hayley Pemble, Lindsay Murrow and Torsten Wittmann

      Part B: Tools

    31. Visualizing Dynamic Activities of Signaling Enzymes using genetically encodable FRET-based biosensors: from designs to applications
    32. Xin Zhou, Katie J. Herbst, Jin Zhang

    33. Live-cell Imaging of Aquaporin-4 Supramolecular Assembly and Diffusion
    34. A.S. Verkman, Andrea Rossi and Jonathan M. Crane

    35. Coiled-coil tag–probe labeling methods for live-cell imaging of membrane receptors
    36. Yoshiaki Yano, Kenichi Kawano, Kaoru Omae, and Katsumi Matsuzaki

    37. Monitoring Protein Interactions in Living Cells with Fluorescence Lifetime Imaging Microscopy
    38. Yuansheng Sun, Nicole M. Hays, Ammasi Periasamy, Michael W. Davidson, and Richard N. Day

    39. Open Source Tools for Fluorescent Imaging
    40. Nicholas A. Hamilton

    41. Nanoparticle PEBBLE sensors in live cells

      Yong-Eun Koo Lee and Raoul Kopelman


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