Human Stem Cell Manual book cover

Human Stem Cell Manual

A Laboratory Guide

This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work.  The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work.

Audience
Scientists engaged in any aspect of stem cell research, as well as undergraduate and graduate students enrolled in laboratory courses.

Spiral Bound, 656 Pages

Published: August 2012

Imprint: Academic Press

ISBN: 978-0-12-385473-5

Contents

  • Foreword by James Battey
    Foreword by Jeannie Fontana
    Preface
    Part I: Basic methods: Maintenance of stem cell cultures
    1.Human Embryonic Stem Cell Culture
    2.Feeder layers and feeder-free culture
    3.Mouse Embryonic Fibroblast Feeder Cells
    4.Cryopreservation of hESC
    Part II: Basic methods: Characterization of stem cells
    5.Classical Cytogenetics: Karyotyping
    6.Spectral Karyotyping and Fluorescent in situ Hybridization
    7.Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns
    8.FACS/Cell sorting
    9.Fluorescence Immunocytochemical Analysis of Stem Cells
    10.Characterization of Stem Cells Using RT-PCR
    11.Gene Expression Profiling of Stem Cells by Microarray
    12.Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice
    13.Generation of HESC-Derived Teratomas
    Part III: Differentiation of stem cells
    14.“Embryoid Body” Formation and Neuroepithelia Differentiation
    15.Motor Neuron and Dopamine Neuron Differentiation
    16.Oligodendrocyte Differentiation from hESC
    17.Cardiac
    18.Hematopoietic
    Part IV: Genetic manipulation of stem cells
    19.Genetic Manipulation of hESC: Lentivirus Vectors
    20.Methods developed for mouse ES cells
    Part V: Advanced methods
    21.Derivation of human ES cells
    22.Neural Stem Cell Culture
    23.Transplantation of stem cells
    24.In vitro fertilization
    25.Functional characterization-neural cells
    Part VI: Practical issues in establishing a human ES cell laboratory
    26.Setting Up a Facility for hESC Research
    Part VII: Essays on stem cell biology
    27.Intellectual Property: Owning the Stem Cell
    28.Ethical Concerns for Stem Cell Research
    29.Guidelines for hESC Research Oversight (ESCRO) Committees

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