High Throughput Bioanalytical Sample Preparation

Methods and Automation Strategies


  • David Wells
  • David Wells, Sample Prep Solutions, St. Paul, Minnesota, USA

High Throughput Bioanalytical Sample Preparation: Methods and Automation Strategies is an authoritative reference on the current state-of-the-art in sample preparation techniques for bioanalysis. This book focuses on high throughput (rapid productivity) techniques and describes exactly how to perform and automate these methodologies, including useful strategies for method development and optimization. A thorough review of the literature is included within each of these chapters describing high throughput sample preparation techniques: protein removal by precipitation; equilibrium dialysis and ultrafiltration; liquid-liquid extraction; solid-phase extraction; and various on-line techniques.The text begins with an introductory overview of the role of bioanalysis in pharmaceutical drug development. Fundamental understanding of the strategies for sample preparation is reinforced next, along with essential concepts in extraction chemistry. Several chapters introduce and discuss microplates, accessory products and automation devices. Particular strategies for efficient use of automation within a bioanalytical laboratory are also presented. The subject material then reviews protein precipitation, liquid-liquid extraction, solid-phase extraction and various on-line sample preparation approaches. The book concludes with information on recent advances in sample preparation, such as solid-phase extraction in a card format and higher density extraction plates.Important objectives that can be accomplished when the strategies presented in this book are followed include: improved efficiency in moving discovery compounds to preclinical status with robust analytical methods; return on investment in automation for sample preparation; and improved knowledge and expertise of laboratory staff.
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Researchers in pharmaceutical drug development, drug analysis and related clinical chemistry disciplines for therapeutic drug monitoring, and forensic scientists who perform bioanalytical sample preparation.


Book information

  • Published: January 2003
  • Imprint: ELSEVIER
  • ISBN: 978-0-444-51029-7


" High Throughout Bioanalytical Sample Preparation is a well written and convenient monograph for anyone involved in sample preparation method development, and gives the reader information that will be useful in daily laboratory work. This book is helpful in the first steps of planning sample throughput for bioanalysis in the field of pharmaceutical drug development."
Hiroyuki Kataoka, School of Pharmacy, Shujitsu University, Japan, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005

"It certainly represents a valuable addition to the literature on sample preparation for bioanalysis and should be read by both experts and beginners in the field."
I.D. Wilson, CHROMATOGRAPHIA, Vol. 61, No. 5/6, 2005

Table of Contents

Chapter 1. Role of Bioanalysis in Pharmaceutical Drug DevelopmentChapter 2. Fundamental Strategies for Bioanalytical Sample PreparationChapter 3. High Throughput Tools for Bioanalysis: MicroplatesChapter 4. High Throughput Tools for Bioanalysis: Accessory ProductsChapter 5. Automation Tools and Strategies for BioanalysisChapter 6. Protein Precipitation: High Throughput Techniques and Strategies for Method DevelopmentChapter 7. Protein Precipitation: Automation StrategiesChapter 8. Liquid-Liquid Extraction: High Throughput TechniquesChapter 9. Liquid-Liquid Extraction: Strategies for Method Development and OptimizationChapter 10. Liquid-Liquid Extraction: Automation StrategiesChapter 11. Solid-Phase Extraction: High Throughput TechniquesChapter 12. Solid-Phase Extraction: Strategies for Method Development and OptimizationChapter 13. Solid-Phase Extraction: Automation StrategiesChapter 14. On-Line Sample Preparation: High Throughput Techniques and Strategies for Method DevelopmentChapter 15. Advances in Sample Preparation for Bioanalysis