High Resolution Separation and Analysis of Biological Macromolecules, Part B: Applications book cover

High Resolution Separation and Analysis of Biological Macromolecules, Part B: Applications

High Resolution Separation and Analysis of Biological Macromolecules, Part B: Applications

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Audience
Biochemists, molecular biologists, biotechnologists, biophysicists, and pharmaceutical chemists.

Included in series
Methods in Enzymology

Hardbound, 555 Pages

Published: July 1996

Imprint: Academic Press

ISBN: 978-0-12-182172-2

Reviews

  • "These two books (volumes 270 and 271) comply with and add to the outstanding reputation of the Methods in Enzymology series as a primary source for detailed and practical information on techniques used in the biological sciences. The excellent, step-by-step description of the techniques described, the clear schematic illustrations, and the frequent addition of technical tricks which are not normally described in the methods sections of scientific journals will facilitate the successful application of the techniques... Together, Methods in Enzymology Volumes 270 and 271 are an invaluable resource for the beginner and the experienced researcher alike."
    Praise for the Volume, --Daniel Figeys and Ruedi Aebersold in ANALYTICAL BIOCHEMISTRY


    "The Methods in Enzymology series represents the gold-standard."
    Praise for the Series, --NEUROSCIENCE
    "Incomparably useful."
    --ANALYTICAL BIOCHEMISTRY
    "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
    --BIO/TECHNOLOGY
    "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
    --CHEMISTRY IN INDUSTRY
    "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
    --AMERICAN SOCIETY OF MICROBIOLOGY NEWS
    "If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
    --ENZYMOLOGIA
    "A series that has established itself as a definitive reference for biochemists."
    --JOURNAL OF CHROMATOGRAPHY

Contents

  • Liquid Chromatography: C.T. Mant and R.S. Hodges, Analysis of Peptides by High-Performance Liquid Chromatography. E.R. Hoff and R.C. Chloupek, Analytical Peptide Mapping of Recombinant DNA-Derived Proteins byReversed-Phase High-Performance Liquid Chromatography. K.M. Swiderek, T.D. Lee, and J.E. Shively, Trace Structural Analysis of Proteins. L.J. Janis, P.M. Kovach, R.M. Riggin, and J.K. Towns, Protein Liquid Chromatographic Analysis in Biotechnology. D. Josic and K. Zeilinger, Membrane Proteins. R.R. Townsend, L.J. Basa, and M.W. Spellman, Identification and Characterization of Glycopeptides in Tryptic Maps by High-pH Anion-Exchange Chromatography. J.R. Thayer, R.M. McCormick, and N. Avdalovic, High Resolution Nucleic Acid Separations by High-Performance Liquid Chromatography. Electrophoresis: Slab Gel Electrophoresis: High Resolution: M.J. Dunn and J.M. Corbett, Two-Dimensional Polyacrylamide Gel Electrophoresis. K. Shimura and K.-i. Kasai, Affinophoresis: Selective Electrophoretic Separation of Proteins Using Specific Carriers. L.M. Smith, R.L. Brumley, Jr., E.C. Buxton, M. Giddings, M. Marchbanks, and X. Tong, High-Speed Automated DNA Sequencing in Ultrathin Slab Gels. Capillary Electrophoresis: E.C. Rickard and J.K. Towns, Applications of Capillary Zone Electrophoresis to Peptide Mapping. G. Teshima and S.-L. Wu, Capillary Electrophoresis Analysis of Recombinant Proteins. B.L. Karger, F. Foret, and J. Berka, Applications of Polymer Matrices to DNA and Protein Analysis by Capillary Electrophoresis. M.V. Novotny, Glycoconjugate Analysis by Capillary Electrophoresis. Mass Spectrometry: J.R. Yates, Protein Structure Analysis by Mass Spectrometry. V.N. Reinhold, B.B. Reinhold, and S. Chan, Carbohydrate Sequence Analysis by Electrospray Ionization–Mass Spectrometry. W.S. Hancock, A. Apffel, J. Chakel, C. Souders, T.M. Timkulu, E. Pungor,Jr., and A.W. Guzzetta, Reversed-Phase Peptide Mapping of Recombinant Human Glycoproteins Using Liquid Chromatography/Electrospray Ionization–Mass Spectrometry. B.L. Gillece-Castro and J.T. Stults, Peptide Characterization by Mass Spectrometry. R.D. Smith, H.R. Udseth, J.H. Wahl, D.R. Goodlett, and S.A. Hofstadler, Capillary Electrophoresis–Mass Spectrometry. J. Frenz, Trace Analysis Peptide Mapping by High-Performance Displacement ChromatographyR.W. Giese, M. Saha, S. Abdel-Baky, and K. Allam, Measuring DNA Adducts by Gas Chromatography–Electron Capture–Mass Spectrometry: Trace Organic Analysis. Author Index. Subject Index.

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