Heterotrimeric G-Protein EffectorsEdited by
- Ravi Iyengar, Mount Sinai School of Medicine, New York, U.S.A.
- John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
- Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, USA
This volume of Methods in Enzymology and its companion Volume 237 include molecular, biological, and biochemical methods for the study of cell surface heterotrimeric G proteins and their effectors. Methods unique to signal transducing G proteins and general techniques that have been applied to the study of G protein systems are covered.
Biochemists, molecular biologists, neurophysiologists,
Methods in Enzymology
Published: September 1994
Imprint: Academic Press
"The Methods in Enzymology series represents the gold-standard."
Praise for the Series , --NEUROSCIENCE
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY
- cGMP Phosphodiesterase:A. Tar, T.D. Ting, and Y.-K. Ho, Purification of Bovine Retinal cGMP Phosphodiesterase.H.M. Rarick, N.O. Artemyev, J.S. Mills, N.P. Skiba, and H.E. Hamm, Specific Peptide Probes for G-Protein Interaction with Effectors. Adenylyl Cyclases:R.A. Johnson, R. Alvarez, and Y. Salomon, Determination of Adenylyl Cyclase Catalytic Activity Using Single and Double Column Procedures.R.A. Johnson and I. Shoshani, Preparation and Use of P-Site-Targeted Affinity Ligands for Adenylyl Cyclases.D.M.F. Cooper, Regulation of Ca2f-Sensitive Adenylyl Cyclases by Calcium in Vitro and in Vivo.Y.H. Wong, Gi Assays in Transfected Cells.R. Taussig, W.-J. Tang, and A.G. Gilman, Expression and Purification of Recombinant Adenylyl Cyclases in Sf9 Cells.O. Jacobowitz, J. Chen, and R. Iyengar, Transient Expression Assays for Mammalian Adenylyl Cyclases.R.T. Premont, Identification of Adenylyl Cyclases by Amplification Using Degenerate Primers. Phospholipases C:M. De Vivo, Assays for G-Protein Regulation of Phospholipase C Activity.E.M. Landau and R.D. Blitzer, Chloride Current Assay for Phospholipase C in XenopusOocytes.S. Cockcroft, G.M.H. Thomas, E. Cunningham, and A. Ball, Use of Cytosol-Depleted HL60 Cells for Reconstitution Studies of G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-( Isozymes.G.M.H. Thomas, E. Cunningham, and S. Cockcroft, Purification of Phosphatidylinositol Transfer Protein from Brain Cytosol for Reconstituting G-Protein-Regulated Phosphoinositide-Specific Phospholipase C-( Isozymes.P. Gierschik and M. Camps, Stimulation of Phospholipase C by G-Protein (( Subunits.G.L. Waldo, A.J. Morris, and T.K. Harden, Purification of G-Protein-Regulated Phospholipase C from Turkey Erythrocytes.A.A. Bominaar and P.J.M. Van Haastert, Phospholipase C Activity in Dictyostelium discoideum Using Endogenous Nonradioactive Phosphatidylinositol 4,5-Bisphosphate as Substrate.D.-Y. Jhon, D. Park, and S.G. Rhee, Purification of Phospholipase C-(3 from Rat Brain.C.-W. Lee, K.-H. Lee, and S.G. Rhee, Characterization of Phospholipase C Isozymes inBovine Retina: Purification of Phospholipase C-(4.J.L. Blank and J.H. Exton, Purification of 110 kDa Phosphoinositide Phospholipase C Activated by G-Protein (( Subunits.H.-W. Ma, R. Iyengar, and R.T. Premont, Amplification of Phosphatidylinositol-Specific Phospholipase C-( Isoforms Using Degenerate Primers.Growth and Transformation:R.R. Vaillancourt, A.E. Harwood, and S. Winitz, Analysis of Guanine Nucleotides Associated with Protooncogene Ras.A.M. Gardner, C.A. Lange-Carter, R.R. Vaillancourt, and G.L. Johnson, Measuring Activation of Kinases in Mitogen-Activated Protein Kinase Regulatory Network.C.A. Hauser, C.J. Der, and A.D. Cox, Transcriptional Activation Analysis of Oncogene Function.A.D. Cox and C.J. Der, Biological Assays for Cellular Transformation. Ions and Channels:S.R. Rawlings, J.-M. Theler, and W. Schlegel, Monitoring of Receptor-Mediated Changes in Intracellular Calcium at the Cellular and Subcellular Levels by Microfluorimetryand Imaging.N. Demaurex, S.R. Rawlings, K.-H. Krause, M.E.E. Jaconi, P.D. Lew, and W. Schlegel, Combination of Microfluorimetric Monitoring of Cytosolic Calcium and pH with Patch Clamp Electrophysiological Recordings in Neutrophil Granulocytes.L. Stehno-Bittel, J. Amundson, and D. Clapham, G-Protein-Mediated Pathways Assayed by Electrophysiology and Confocal Microscopy.D.R. Witcher, M. De Waard, S.D. Kahl, and K.P. Campbell, Purification and Reconstitution of N-Type Calcium Channel Complex from Rabbit Brain.R. Andrade, Infusion of Guanine Nucleotides through Recording Electrodes for Studies on G-Protein Regulation of Ion Currents and Channels.I. McFadzean, M.P. Caulfield, Y. Vallis, and D.A. Brown, Injection of Antiserainto Cells to Study G-Protein Regulation of Channel Function.J. Hescheler, Whole-Cell Clamp Analysis for G-Protein Regulation of Channels.R.D. Blitzer and E.M. Landau, Whole-Cell Patch Recording in Brain Slices.A. Yatani, Reconstitution of Receptor-Regulated Ion Channels in Isolated Patch Membrane.M. Yamada, A. Terzic, and Y. Kurachi, Regulation of Potassium Channels by G-Protein Subunits and Arachidonic Acid Metabolites.Author Index.Subject Index.