Handbook of Isoelectric Focusing and Proteomics

Edited by

  • David Garfin, Garfin Consulting, Kensington, California, USA
  • Satinder Ahuja, Ahuja Consulting, Calabash, NC, USA

Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics.
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Audience

Students and researchers in global academic research in health science institutes and the pharmaceutical industry

 

Book information

  • Published: May 2005
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-088752-1


Table of Contents

Preface (D. Garfin, S. Ahuja).
Incidents of Travel in IEF and IPGs (P. Giorgio Righetti).
Evolution and Development of IEP (A. Vegvari, F. Kilar).
Theory and Simulation of IEF (T. L. Sounart et al.)
Generation of pH Gradients (T. Berkelman).
Slab Gel IEF (R. Westermeier).
Two-Dimensional Gel Electrophoresis (M.P. Mollay et al.).
Some Practices and Pitfalls of Sample Preparation for IEF in Proeomics (B. Herbert).
Protein Detection and Imaging in IEF Gels (W.P. Patton).
Capillary IEF (T. Wehr).
Free Flow IEF (P.J.A. Weber et al.).
Isoelectric Focusing and Proteomics (S.J. Cordwell, M.Y. White).
Chromatofocusing (D. Anderson).
Alternative Forms of IEF (C.F. Ivory).