Guide to Techniques in Mouse Development, Part B book cover

Guide to Techniques in Mouse Development, Part B

Mouse Molecular Genetics

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.  


Researchers and students in biochemistry, cardiology, cell and molecular biology, neuroscience, pharmacology, endocrinology.

Included in series
Methods in Enzymology

Hardbound, 628 Pages

Published: August 2010

Imprint: Academic Press

ISBN: 978-0-12-384880-2


  • Section I. Transgenesis

    1. Lentivirus transgenesis

    Alexander Pfeifer and Katrin Zimmermann

    2. Germline modification using mouse spermatogonial stem cells

    Mito Kanatsu-Shinohara and Takashi Shinohara

    3. Embryonic in vivo electroporation in the mouse

    Tetsuichiro Saito

    Section II. Transposons

    4. Current applications of transposons in mouse genetics

    Adam J. Dupuy

    5. Functional genomics in the mouse using the Sleeping Beauty transposon system

    Kyoji Horie, Chikara Kokubu, and Junji Takeda

    6. The use of DNA transposons for cancer gene discovery in mice

    George Vassiliou, Roland Rad, and Allan Bradley


    Section III. Recombinases

    7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2

    Konstantinos Anastassiadis, Stefan Glaser, Andrea Kranz, Kaj Berhardt, and A. Francis Stewart

    8. A recombineering pipeline to make conditional targeting constructs

    Jun Fu, Madeleine Teucher, Konstantinos Anastassiadis, William Skarnes, and A. Francis Stewart

    9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria

    M. David Stewart and Richard R. Behringer

    10.Genetic fate mapping using site-specific recombinases

    Emilie Legué and Alexandra L. Joyner

    11. Mapping cell fate and function using recombinase-based intersectional strategies

    Susan M. Dymecki, Russell S. Ray, and Jun C. Kim


    Section IV. Mutagenesis

    12. Genome-wide forward genetic screens in mouse ES cells

    Meng Amy Li, Stephen J. Pettitt, Kosuke Yusa, and Allan Bradley

    13. Gene trap mutagenesis in the mouse

    Roland H. Friedel and Philippe Soriano

    14. A wider context for gene trap mutagenesis

    Joshua M. Brickman, Anestis Tsakiridis, Christine To, and William L. Stanford

    15. Mouse mutagenesis with the chemical supermutagen ENU

    Frank J. Probst and Monica J. Justice

    16. Phenotype-driven mouse ENU mutagenesis screens

    Tamara Caspary

    17. Using ENU mutagenesis for phenotype-driven analysis of the mouse.

    Rolf W. Stottmann and David R. Beier


    Section V. Gene Knockdowns

    18. Exploration of self-renewal and pluripotency in ES cells using RNAi

    Christoph Schaniel, Dung-Fang Lee, and Ihor R. Lemischka

    19. Transgenic RNAi applications in the mouse

    Jost Seibler and Frieder Schwenk

    20. Gene knockdown in the mouse through RNAi

    Aljoscha Kleinhammer, Wolfgang Wurst, and Ralf Kühn

    21. In vivo analysis of gene knock-down in tetracycline-inducible shRNA mice

    Christopher S. Raymond, Lei Zhu, and Myung K. Shin

    22. The power of reversibility: Regulating gene activities via tetracycline controlled transcription

    Kai Schönig, Hermann Bujard, and Manfred Gossen

    Section VI. Gene Expression Profiling

    23. Gene expression profiling of mouse oocytes and preimplantation embryos

    Francesca E. Duncan and Richard M. Schultz

    24. Interrogating the transcriptome of oocytes and preimplantation embryos

    Anne E. Peaston, Joel Graber, Barbara B. Knowles, and Wilhelmine N. de Vries

    25. Gene expression profiling of mouse embryos with microarrays

    Alexei A. Sharov, Yulan Piao, and Minoru S. H. Ko


advert image