Guide to Protein Purification
Volume 182: Guide to Protein Purification
- Murray Deutscher, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.
- John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
- Melvin Simon, The Salk Institute, La Jolla, CA, USA
Protein biochemists, enzymologists, analytical biochemists, graduate students, and postdoctorates in these disciplines, cell and molecular biologists, biotechnologists, and industrial researchers working with protein products.
- Published: February 1990
- Imprint: ACADEMIC PRESS
- ISBN: 978-0-12-182083-1
"Packed within this volume is much of the accumulated practical wisdom of a century of biochemistry. When available at the bench, it should become widely used for answering the endless questions that arise in the research laboratory. The information is generous in detail and accompanied by sage advice from sixty-nine contributors who share the lessons of their wide experience. The subject matter is in logical sequence, with early presentation of general information for handling proteins that includes an immensely useful table of fifty-four buffers, a discussion of seven different methods for measuring protein quantity, ten pages on maintenance of protein stability, a large section on solubilization, two contributions on overexpression, and much more. Preparation of extracts from the full spectrum of cell and organelle types used in biochemistry is covered by eight authors. About one-third of the book is devoted directly to purification procedures, from ammonium sulfate precipitation methods of the last century to the most advanced chromatographic and electrophoretic methods of today. A large selection is concerned with the characterization of purified proteins, and includes a computerized interpretation of physical and analytical data. The book has much else to recommend it in its readability and thoughtful commentary. In a discussion on
Praise for the Volume, --Simon Black in ANALYTICAL BIOCHEMISTRY
"The freshness of Arthur Kornbergs introductory prose... takes the mundane and often frankly tedious business of isolating proteins onto another plane: 'Don't waste clean thinking on dirty enzymes'. In the epilogue, other giants of biochemistry remind us of the inherited wealth (often taken for granted) that we possess through the study of purified proteins. Strong stuff for a practical guide. But then this is more than just a guide. It is a complete work. The strategies described embody both classical and modern thinking. We are told what to do to set up a separation laboratory in classic terms (don't start with wall-to-wall FPLC). New biochemists sometimes neglect their roots in physical chemistry. The chapters on buffers, protein assay, and quantitation will repay study. The editor has recognized that the everyday problems are protein desalting, concentration, recovery and storage. The advice here is comprehensive. In summary, this is possibly the most important, comprehensive and affordable work on protein purification to have appeared in recent years. Librarians who want to retain it as a reference work will have to put it behind glass and turn a page each day. This reviewer is going to keep his copy at home."
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