Browse books > Guide to Protein Purification

Guide to Protein Purification

Edited by
  • Richard Burgess
  • Murray Deutscher, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.

Included in series
Methods in Enzymology

Hardbound, 900 Pages

Published: November 2009

Imprint: Academic Press

ISBN: 978-0-12-374536-1

Contents

    1. Why Purify Enzymes?

      2. Murray Deutscher and Arthur Kornberg

      Section I: Developing Purification Procedures

    2. Strategies and Considerations for Protein Purifications

      3. Stuart Linn

    3. Use of Bioinformatics in Planning a Protein Purification

      4. Richard R. Burgess

    4. Preparing A Purification Summary Table

      5. Richard R. Burgess

      Section II: General Methods for Handling Proteins and Enzymes

    5. Setting Up A Laboratory

      6. Murray P. Deutscher

    6. Buffers: Principle and Practice

      7. Vincent S. Stoll and John S. Blanchard

    7. Measurement of Enzyme Activity

      8. T. K. Harris and M. M. Keshwani

    8. Quantitation of Protein

      9. James E. Noble and Marc J. A. Bailey

    9. Concentration of Proteins and Removal of Solutes

      10. Jonathan K. Romero, David R. H. Evans and Matthew Westoby

    10. Maintaining Protein Stability

      11. Murray P. Deutscher

      Section III: Recombinant Protein Expression and Purification

    11. Selecting an Appropriate Method for Expressing a Recombinant Protein

      12. William H. Brondyk

    12. Bacterial Systems for Production of Heterologous Proteins

      13. Frank R. Collart, Ashley M. Frank and Sarah Zerbs

    13. Expression in the Yeast Pichia Pastoris

      14. James M. Cregg, Ilya Tolstorukov, Anasua Kusari, Jay Sunga, Knut Madden and Thomas Chappell

    14. Baculovirus-Insect Cell Expression Systems

      15. Donald L. Jarvis

    15. Recombinant Protein Production By Transient Gene Transfer Into Mammalian Cells

      16. Sabine Geisse and Cornelia Fux

    16. Tagging For Protein Expression

      17. Arun Malhotra

    17. Refolding of Solubilized Inclusion Body Proteins

      18. Richard R. Burgess

      Section IV: Preparation of Extracts and Subcellular Fractionation

    18. Advances In Preparation of Biological Extracts for Protein Purification

      19. Anthony C. Grabski

    19. Isolation of Subcellular Organelles and Structures

      20. Jörg Von Hagen and Uwe Michelsen

      Section V: Purification Procedures: Bulk Methods

    20. Protein Precipitation Techniques

      21. Richard R. Burgess

    21. Affi-Gel Blue for Nucleic Acid Removal and Early Enrichment of Nucleotide Binding Proteins

      22. Murray P. Deutscher

      Section VI: Purification Procedures: Chromatographic Methods

    22. Ion Exchange Chromatography

      23. Alois Jungbauer and Rainer Hahn

    23. Gel Filtration

      24. Earle Stellwagen

    24. Protein Chromatography on Hydroxyapatite Columns

      25. Larry J. Cummings, Mark A. Snyder and Kimberly Brisack

    25. Theory and Use of Hydrophobic Interaction Chromatography in Protein Purification Applications

      26. Justin T. Mccue

      Section VII: Purification Procedures: Affininty Methods

    26. Affinity Chromatography: General Methods

      27. Marjeta Urh, Dan Simpson and Kate Zhao

    27. Immobilized-Metal Affinity Chromatography (IMAC)

      28. Frank Schäfer* Helena Block, Barbara Maertens, Anne Spriestersbach, Nicole Brinker, Jan Kubicek, Roland Fabis And Jörg Labahn

    28. Identification, Production, and Use of Polyol-Responsive Monoclonal Antibodies for Immunoaffinity Chromatography

      29. Nancy E. Thompson, Katherine M. Foley, Elizabeth S. Stalder, And Richard R. Burgess

      Section VIII: Purification Procedures: Electrophoretic Methods

    29. One-Dimensional Gel Electrophoresis

      30. David E. Garfin

    30. Protein Analysis Using High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis and Isoelectric Focusing

      31. Friedman

    31. Protein Gel Staining Methods: An Introduction And Overview

      32. Thomas H. Steinberg

    32. Elution of Proteins From Gels

      33. Richard R. Burgess

    33. Immunodetection By Protein Blotting Performing and Optimizing Western Blots With An Emphasis on Chemiluminescent Detection

      34. Alice Alegria-Schaffer, Krishna Vattem and Andrew Lodge

      Section IX: Purification Procedures: Membrane Proteins And Glycoproteins

    34. Detergents: An Overview

      35. Dirk Linke

    35. Purification of Membrane Proteins

      36. Sue-Hwa Lin and Guido Guidotti

    36. Purification of Recombinant G-Protein-Coupled Receptors

      37. Reinhard Grisshammer

    37. Cell-Free Translation Of Integral Membrane Proteins Into Unilamelar Liposomes

      38. Michael A. Goren, Akira Nozawa, Shin-Ichi Makino, Russell L. Wrobel and Brian G. Fox

      Section X: Characterization of Purified Proteins

    38. Determination of Protein Purity

      39. David G. Rhodes and Thomas M. Laue

    39. Determination of Size, Molecular Weight, and Presence of Subunits

      40. David G. Rhodes, Robert E. Bossio and Thomas M. Laue

    40. Identification and Quantification of Protein Posttranslational Modifications

      41. Andrew Link

    41. Parallel Methods For Expression And Purification

      42. Scott A. Lesley

    42. Techniques To Isolate O2-Sensitive Proteins: [4Fe-4S]-FNR As An Example

      43. Aixin Yan and Patricia J. Kiley

    43. Rethinking Your Purification Procedure

      44. Murray Deutscher

    44. Important But Little Known (Or Forgotten) Artifacts in Protein Biochemistry

    Richard R. Burgess

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