Glycoprotein and Proteoglycan TechniquesBy
- J.G. Beeley
The past two decades have seen an expansion of interest in glycoproteins. From being a borderline area between carbohydrate and protein chemistry, it has become relevant to a wide range of biological phenomena. The aim of the book is to describe techniques which can be used to answer some of the basic questions about glycosylated proteins. Methods are discussed for isolation; compositional analysis; structure carbohydrate units; protein-carbohydrate linkages, keeping in mind the diverse nature of problems which readers may have to tackle.
Laboratory Techniques in Biochemistry and Molecular Biology
Published: January 1985
..... it gives a good, broad perspective on the variable and yet ordered structures shown by glycoproteins and proteoglycans, and a thorough chemical overview of what can and cannot be done to the intact glycoproteins and their isolated glycopeptides.
- 1. Introduction. 2. Glycoproteins and proteoglycans. 3. Isolation and fractionation. 4. Physico-chemical characterisation. 5. Analysis of constituents. 6. Structural analysis. 7. Lectin techniques. 8. Radioactive labelling techniques. Appendix. References. Index.