Ghrelin book cover

Ghrelin

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers ghrelin, and has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice.

Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 512 Pages

Published: September 2012

Imprint: Academic Press

ISBN: 978-0-12-381272-8

Contents

  • History of GHS and ghrelin
    1. History To The Discovery Of Ghrelin
      Cyril Y. Bowers

    2. Orphan Gpcrs And Methods For Identifying Their Ligands
      Morikatsu Yoshida, Mikiya Miyazato, and Kenji Kangawa

    3. Purification Of Rat And Human Ghrelins
      Masayasu Kojima, Hiroshi Hosoda and Kenji Kangawa

    4. Variety of Acyl Modifications in Mammalian Ghrelins
      Takanori Ida

    5. Determination Of Non-Mammalian Ghrelin
      Hiroyuki Kaiya, Hiroshi Hosoda, Kenji Kangawa, Mikiya Miyazato

      Molecular forms of ghrelin and Measuring of the concentrations

    6. Morphological Analysis Of Ghrelin Neurons In The Hypothalamus
      Haruaki Kageyama, Fumiko Takenoya and Seiji Shioda

    7. High-Performance Liquid Chromatography Analysis Of Hypothalamic Ghrelin
      Takahiro Sato and Masayasu Kojima

    8. Standard Sample Collections For Blood Ghrelin Measurements
      Hiroshi Hosoda and Kenji Kangawa

      Enzymes for processing of ghrelin

    9. From Ghrelin to Ghrelin’s O Acyl Transferase
      Jesus A. Gutierrez, Jill A. Willency, Michael D. Knierman, Tamer Coskun, Patricia J. Solenberg, Doug R. Perkins, Richard E. Higgs, and John E. Hale.

    10. Enzymatic Characterization Of Goat, Ghrelin O-Acyltransferase
      Hideko Ohgusu, Tomoko Takahashi and Masayasu Kojima

    11. The Study Of Ghrelin Deacylation Enzymes
      Motoyasu Satou and Hiroyuki Sugimoto

      Synthesis of ghrelin agonists and antagonists

    12. Synthesis?Of Ghrelin?Chemical Synthesis And Semi-Synthesis For Large-Scale Preparation Of Modified Peptides
      Tomohiro Makino, Masaru Matsumoto and Yoshiharu Minamitake

    13. Ghrelin O-Acyltransferase Assays And Inhibition
      Martin S. Taylor, Yousang Hwang, Po-Yuan Hsiao, Jef D. Boeke, Philip A. Cole

      Action of ghrelin

    14. Model-Based Evaluation Of Growth Hormone Secretion
      Johannes D. Veldhuis, Daniel M. Keenan

    15. Ghrelin In The Control Of Energy, Lipid And Glucose Metabolism
      Kristy M. Heppner, Timo D. Müller, Jenny Tong, and Matthias H. Tschöp

    16. Ghrelin And The Vagus Nerve
      Yukari Date

    17. Measurement of amp-activated protein kinase (ampk) activity and expression In response to ghrelin
      Chung Thong Lim, Francesca Lolli, Julia D Thomas, Blerina Kola, Márta Korbonits

    18. Ghrelin And Gastrointestinal Movement
      Naoki Fujitsuka, Akihiro Asakawa, Haruka Amitani, Mineko Fujimiya, Akio Inui

    19. Ghrelin Acylation By Ingestion Of Medium-Chain Fatty Acids
      Yoshihiro Nishi, Hiroharu Mifune, and Mayasu Kojima

    20. Islet ß-cell Ghrelin Signaling For Inhibition Of Insulin Secretion
      Katsuya Dezaki & Toshihiko Yada

    21. Rikkunshito As A Ghrelin Enhancer
      Hiroshi Takeda, Shuichi Muto, Koji Nakagawa, Shunsuke Ohnishi, Chiharu Sadakane, Yayoi Saegusa, Miwa Nahata, Tomohisa Hattori and Masahiro Asaka

      Tg and KO mice of ghrelin

    22. Thermogenic Characterization Of Ghrelin Receptor Null Mice
      Ligen Lin and Yuxiang Sun

    23. Transgenic Mice Over-Expressing Ghrelin Or Ghrelin Analog
      Hiroyuki Ariyasu, Go Yamada, Hiroshi Iwakura, Takashi Akamizu, Kenji Kangawa and Kazuwa Nakao

      Clinical application of ghrelin

    24. Therapeutic Potential Of Ghrelin In Restricting-Type Anorexia Nervosa
      Mari Hotta, Rina Ohwada, Takashi Akamizu, Tamotsu Shibasaki, Kenji Kangawa

    25. Clinical Application Of Ghrelin For Chronic Respiratory Diseases
      Nobuhiro Matsumoto, Masamitsu Nakazato

    26. Clinical Trial Of Ghrelin Synthesis Administration For Upper Gi Surgery
      Shuji Takiguchi, Yuichiro Hiura, Yasuhiro Miyazaki, Akihiro Takata, and Yuichoro Doki

Advertisement

advert image