Ghrelin

Edited by

  • John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers ghrelin, and has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice.
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Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

 

Book information

  • Published: September 2012
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-381272-8


Table of Contents

History of GHS and ghrelin

  1. History To The Discovery Of Ghrelin
  2. Cyril Y. Bowers

  3. Orphan Gpcrs And Methods For Identifying Their Ligands
  4. Morikatsu Yoshida, Mikiya Miyazato, and Kenji Kangawa

  5. Purification Of Rat And Human Ghrelins
  6. Masayasu Kojima, Hiroshi Hosoda and Kenji Kangawa

  7. Variety of Acyl Modifications in Mammalian Ghrelins
  8. Takanori Ida

  9. Determination Of Non-Mammalian Ghrelin
  10. Hiroyuki Kaiya, Hiroshi Hosoda, Kenji Kangawa, Mikiya Miyazato

    Molecular forms of ghrelin and Measuring of the concentrations

  11. Morphological Analysis Of Ghrelin Neurons In The Hypothalamus
  12. Haruaki Kageyama, Fumiko Takenoya and Seiji Shioda

  13. High-Performance Liquid Chromatography Analysis Of Hypothalamic Ghrelin
  14. Takahiro Sato and Masayasu Kojima

  15. Standard Sample Collections For Blood Ghrelin Measurements
  16. Hiroshi Hosoda and Kenji Kangawa

    Enzymes for processing of ghrelin

  17. From Ghrelin to Ghrelin’s O Acyl Transferase
  18. Jesus A. Gutierrez, Jill A. Willency, Michael D. Knierman, Tamer Coskun, Patricia J. Solenberg, Doug R. Perkins, Richard E. Higgs, and John E. Hale.

  19. Enzymatic Characterization Of Goat, Ghrelin O-Acyltransferase
  20. Hideko Ohgusu, Tomoko Takahashi and Masayasu Kojima

  21. The Study Of Ghrelin Deacylation Enzymes
  22. Motoyasu Satou and Hiroyuki Sugimoto

    Synthesis of ghrelin agonists and antagonists

  23. SynthesisOf Ghrelin:Chemical Synthesis And Semi-Synthesis For Large-Scale Preparation Of Modified Peptides
  24. Tomohiro Makino, Masaru Matsumoto and Yoshiharu Minamitake

  25. Ghrelin O-Acyltransferase Assays And Inhibition
  26. Martin S. Taylor, Yousang Hwang, Po-Yuan Hsiao, Jef D. Boeke, Philip A. Cole

    Action of ghrelin

  27. Model-Based Evaluation Of Growth Hormone Secretion
  28. Johannes D. Veldhuis, Daniel M. Keenan

  29. Ghrelin In The Control Of Energy, Lipid And Glucose Metabolism
  30. Kristy M. Heppner, Timo D. Müller, Jenny Tong, and Matthias H. Tschöp

  31. Ghrelin And The Vagus Nerve
  32. Yukari Date

  33. Measurement of amp-activated protein kinase (ampk) activity and expression In response to ghrelin
  34. Chung Thong Lim, Francesca Lolli, Julia D Thomas, Blerina Kola, Márta Korbonits

  35. Ghrelin And Gastrointestinal Movement
  36. Naoki Fujitsuka, Akihiro Asakawa, Haruka Amitani, Mineko Fujimiya, Akio Inui

  37. Ghrelin Acylation By Ingestion Of Medium-Chain Fatty Acids
  38. Yoshihiro Nishi, Hiroharu Mifune, and Mayasu Kojima

  39. Islet ß-cell Ghrelin Signaling For Inhibition Of Insulin Secretion
  40. Katsuya Dezaki & Toshihiko Yada

  41. Rikkunshito As A Ghrelin Enhancer
  42. Hiroshi Takeda, Shuichi Muto, Koji Nakagawa, Shunsuke Ohnishi, Chiharu Sadakane, Yayoi Saegusa, Miwa Nahata, Tomohisa Hattori and Masahiro Asaka

    Tg and KO mice of ghrelin

  43. Thermogenic Characterization Of Ghrelin Receptor Null Mice
  44. Ligen Lin and Yuxiang Sun

  45. Transgenic Mice Over-Expressing Ghrelin Or Ghrelin Analog
  46. Hiroyuki Ariyasu, Go Yamada, Hiroshi Iwakura, Takashi Akamizu, Kenji Kangawa and Kazuwa Nakao

    Clinical application of ghrelin

  47. Therapeutic Potential Of Ghrelin In Restricting-Type Anorexia Nervosa
  48. Mari Hotta, Rina Ohwada, Takashi Akamizu, Tamotsu Shibasaki, Kenji Kangawa

  49. Clinical Application Of Ghrelin For Chronic Respiratory Diseases
  50. Nobuhiro Matsumoto, Masamitsu Nakazato

  51. Clinical Trial Of Ghrelin Synthesis Administration For Upper Gi Surgery

Shuji Takiguchi, Yuichiro Hiura, Yasuhiro Miyazaki, Akihiro Takata, and Yuichoro Doki