G Protein Coupled Receptors

Trafficking and Oligomerization

Edited by

  • P. Michael Conn, Texas Tech University Health Sciences Center, Lubbock, USA

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high throughput assays for identification of pharmacoperone drugs.
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Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

 

Book information

  • Published: February 2013
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-391862-8


Table of Contents

    GPCR Trafficking

    1. Therapeutic Rescue of Misfolded/Mistrafficked Mutants: Automation-Friendly High Throughput Assays for Identification of Pharmacoperone Drugs of GPCRs
    2. David C. Smithson, Jo Ann Janovick, P. Michael Conn

    3. Trafficking of the Follitropin Receptor
    4. Alfredo Ulloa-Aguirre, James A. Dias, George Bousfield, Ilpo Huhtaniemi, and Eric Reiter

    5. Single Molecule Imaging Technique to Study the Dynamic Regulation of GPCR Function at the Plasma Membrane
    6. B.E. Snaar-Jagalska, A. Cambi, T. Schmidt and S. de Keijzer

    7. GPCR Oligomerization and Receptor Trafficking
    8. Richard J. Ward, Tian-Rui Xu and Graeme Milligan

    9. b-Arrestins and G Protein-Coupled Receptor Trafficking
    10. Dong Soo Kang, Xufan Tian, and Jeffrey L. Benovic

    11. Tracking Cell Surface Mobility of GPCRs Using α-Bungarotoxin Linked Fluorophores
    12. Saad Hannan, Megan E Wilkins, P Thomas and Trevor G Smart

    13. Regulatory Mechanism of G Protein-Coupled Receptor Trafficking to the Plasma Membrane: A Role for mRNA Localization
    14. Kusumam Joseph, Eleanor K. Spicer and Baby G. Tholanikunnel

    15. Dissecting Trafficking and Signaling of Atypical Chemokine Receptors
    16. Elena Borroni, Cinzia Cancellieri, Massimo Locati, Raffaella Bonecchi


      Trafficking Motifs

    17. Systematic and Quantitative Analysis of G Protein-Coupled Receptor Trafficking Motifs
    18. Carl M. Hurt, Vincent K. Ho, and Timothy Angelotti

    19. Identification of Endoplasmic Reticulum Export Motifs for G Protein-Coupled Receptors
    20. Guangyu Wu

    21. Amino Acid Residues of G-Protein Coupled Receptors Critical for Endoplasmic Reticulum Export and Trafficking
    22. Motonao Nakamura, Daisuke Yasuda, Nobuaki Hirota, Teruyasu Yamamoto, Satoshi Yamaguchi, Takao Shimizu and Teruyuki Nagamune

      GPCR Oligomerization

    23. G Protein-Coupled Heteromers Regulation in Disease
    24. Ivone Gomes, Achla Gupta and Lakshmi A. Devi

    25. Hetero-Oligomerization and Specificity Changes of G Protein-Coupled Purinergic Receptors: Novel Insight into Diversification of Signal Transduction
    26. Tokiko Suzuki, Kazunori Namba, Natsumi Mizuno and Hiroyasu Nakata

    27. Bimolecular Fluorescence Complementation Analysis of G Protein-Coupled Receptor Dimerization in Living Cells
    28. Karin F.K. Ejendal, Jason M. Conley, Chang-Deng Hu, and Val J. Watts

    29. G-Protein Coupled Receptor Heterodimerization in the Brain
    30. Dasiel O. Borroto-Escuela, Wilber Romero-Fernandez, Pere Garriga, Francisco Ciruela, Manuel Narvaez, Alexander O. Tarakanov, Miklós Palkovits, Luigi F. Agnati and Kjell Fuxe

    31. Experimental Strategies for Studying G Protein-Coupled Receptor Homo- and Heteromerization with Radioligand Binding and Signal Transduction Methods
    32. Roberto Maggio, Cristina Rocchi, and Marco Scarselli

    33. Analysis of GPCRs Dimerization Using Acceptor Photobleaching Resonance Energy Transfer Techniques
    34. Marta Busnelli, Mario Mauri, Marco Parenti and Bice Chini

    35. Techniques for the Discovery of GPCR-Associated Protein Complexes
    36. Avais Daulat , Pascal Maurice and Ralf Jockers

    37. Expression, Purification and Analysis of GProtein-Coupled Receptor Kinases
    38. Rachel Sterne-Marr, Alison I. Baillargeon, Kevin R. Michalski, and John J. G. Tesmer

    39. Modern Methods to Investigate the Oligomerization of Glycoprotein Hormone Receptors (TSHR,LHR, FSHR)

    Marco Bonomi and Luca Persani