G Protein Coupled Receptors


Edited by

  • P. Michael Conn, Texas Tech University Health Sciences Center, Lubbock, USA

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain.
View full description


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Book information

  • Published: February 2013
  • ISBN: 978-0-12-391861-1

Table of Contents

    1. Expression of GPCRs in Pichia pastoris for structural studies

      Christoph Krettler, Christoph Reinhart and Carville G. Bevans

    2. Comformational Ensemble View of G Protein-Coupled Receptors and The Effect of Mutations and Ligand Binding
    3. Ravinder Abrol, Soo-Kyung Kim, Jenelle K. Bray, Bartosz Trzaskowski, and William A. Goddard III

    4. Structural Evolution of G-Protein-Coupled Receptors: A Sequence Space Approach
    5. Jean-Michel Bécu, Julien Pelé, Patrice Rodien, Hervé Abdi and Marie Chabbert

    6. Directed Evolution of G Protein-Coupled Receptors for High Functional Expression and Detergent Stability
    7. Karola M. Schlinkmann and Andreas PlĂĽckthun

    8. The Role of Hydrophobic Amino Acids in the Structure and Function of the Rhodopsin Family of G Protein-Coupled Receptors
    9. Gianluigi Caltabiano, Angel González, Arnau Cordomí, Mercedes Campillo, and Leonardo Pardo

    10. Structure of β-Adrenergic Receptors
    11. Florian Brueckner, Chayne L. Piscitelli, Ching-Ju Tsai, Jörg Standfuss, Xavier Deupi and Gebhard F.X. Schertler

    12. Advances in Methods to Characterize Ligand-Induced Ionic Lock and Rotamer Toggle Molecular Switch in G-Protein Coupled Receptors
    13. Xiang-Qun Xie and Ananda Chowdhury

    14. Crystallogenesis of Adenosine A2A Receptor – T4 Lysozyme Fusion Protein: A Practical Route for the Structure
    15. Yashwanth Ashok, Rahul T. Nanekar & Veli-Pekka Jaakola

    16. Probing GPCR Structure: Adenosine and P2Y Nucleotide Receptors
    17. Kenneth A. Jacobson, Stefano Costanzi, and Francesca Deflorian

    18. Strategies for Studying the Ligand Binding Site of GPCRs: Photoaffinity Labeling of the VPAC1 Receptor, a Prototype of Class B GPCRs
    19. Alain Couvineau, Yossan-Var Tan, Emilie Ceraudo and Marc Laburthe

    20. Expression of Mammalian G Protein-Coupled Receptors in Caenorhabditis Elegans
    21. Beata Jastrzebska, David Salom, Hui Jin, Pengxiu Cao, Wenyu Sun, Krzysztof Palczewski, and Zhaoyang Feng

    22. Expression, Purification and Structural Analysis of Intracellular C-Termini from Metabotropic Glutamate Receptors
    23. Angela Seebahn, Heinrich Sticht and Ralf Enz

    24. Unnatural Amino Acid Mutagenesis of GPCRs Using Amber Codon Suppression and Bioorthogonal Labeling
    25. Thomas Huber, Saranga Naganathan, He Tian, Shixin Ye, and Thomas P. Sakmar

    26. Mapping a Ligand Binding Site Using Genetically-Encoded Photoactivatable Crosslinkers
    27. Amy Grunbeck, Thomas Huber, Thomas P. Sakmar

    28. Alternative mRNA Splicing of G Protein-Coulped Receptors
    29. Danijela Markovic

    30. Functional Residues of the CB1 Cannabinoid Receptor

    Joong-Youn Shim and Lea Padgett