Functional Microbial Genomics

Edited by

  • Brendan Wren, London School of Hygiene & Tropical Medicine, U.K.
  • Nick Dorrell, London School of Hygiene and Tropical Medicine, UK

Functional Microbial Genomics, edited by two leading experts in the field, provides the researcher with an up-to-date collection of articles on post-genome technologies central to studying the function of microorganisms.Since the release of the first complete genome sequence of a free-living organism in 1995, over 100 microbial genomes have been completely sequenced. The advent of new technologies for post-genomic analyses has allowed the rapid exploitation of this genome sequence information, heralding a golden era in microbial research. Functional Microbial Genomics provides in-depth accounts from scientists working with these new technologies explaining both the techniques and the ways in which they have been applied to the study of gene function in different microbial species.Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.
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Micro and molecular biologists, geneticists, parasitologists, virologists, pharmacologists, biotechnologists


Book information

  • Published: December 2002
  • ISBN: 978-0-12-521533-6


Praise for the Series: "An invaluable resource for the detailed analysis of cellular gene function and genome architecture." —NATURE

Table of Contents

GENOME SEQUENCE ANALYSISAnnotation of genomes From sequence to consequence: in silico hypothesis generation and testing The atlas visualisation of genome-wide informationCONSTRUCTION OF DNA MICROARRAYSMicroarray design for bacterial genomesGlass slides microarrays for bacterial genomesCOMPARAIVE NUCLEIC ACID ANALYSIS Representational display analysis of Cdna and genome comparisonsApplication of DNA microarrays for comparative and evolutionary genomicsGene expression during host-pathogen interactions: approaches to bacterial mRNA extraction and labelling for microarray analysisHigh throughput in vivo screens: signature-tagged mutagenesisFurther strategies for signature-tagged mutagenesis and the application of oligonucleotide microarrays for the quantitation of DNA-tagged strainsPROTEOME ANALYSISAdvances in bacterial proteome analysisDiscovery of protein-protein interaction using two-hybrid systemsSECTION V APPLICATIONS OF MICROBIAL GENOMICS Cloning the metagenome: Culture-independent access to the diversity and functions of the uncultivated microbial worldReverse vaccinology: from genome to vaccineMicrobial genomics for antibiotic target discovery CASE STUDIES - BACTERIAHelicobacter pylori functional genomics Streptomyces coelicolor A3(2): from genome sequence to functionFunctional analysis of the Bacillus subtilis genomeSECTION VII CASE STUDIES - PARASITESPlasmodium falciparum DNA microarrays and interpretation of dataFunctional analysis of the Plasmodium falciparum genome using transfectionChromosome fragmentation as an approach to whole genome analysis in trypanosomes