Forensic DNA Biology book cover

Forensic DNA Biology

A Laboratory Manual

DNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DNA analysis, including pipetting, microscopy and hair analysis, presumptive testing of body fluids and human DNA typing. Modern DNA typing techniques are provided, reflecting real life, where not all institutions and crime labs can afford the same equipment and software. Real case studies will be used throughout and the author will provide an Instructor Manual and PowerPoint lecture slides.

Audience

Upper-level, undergraduate or graduate students majoring in forensic science, chemistry or biology, academia and newly practicing forensic scientists.

Spiral Bound, 224 Pages

Published: September 2012

Imprint: Academic Press

ISBN: 978-0-12-394585-3

Reviews

  • "This manual presents a collection of novel laboratory experiments intended for advanced undergraduate students and those new to the field of forensic DNA biology and DNA typing."--Evidence Technology Magazine, July-August 2013


Contents

  • 1. Pipetting
    2. Serology
    3. Sampling Biological Evidence for DNA Extraction
    4. DNA Extraction
    5. Determination of quality and quantity of DNA using agarose gel electrophoresis
    6. Determination of DNA quality and quantity using UV-vis spectroscopy
    7. Determination of DNA quantity by fluorescence spectroscopy
    8. Real-time polymerase chain reaction (PCR) quantitation of DNA
    9. Multiplex polymerase chain reaction (PCR) primer design (in silico)
    10. Testing designed PCR primers in multiplex reactions
    11. Multiplex polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci using a commercial kit
    12. Capillary electrophoresis of short-tandem repeat (STR) polymerase chain reaction (PCR) products from a commercial multiplex kit
    13. Computing random match probability from DNA profile  data using population databases
    14. Mitochondrial deoxyribonucleic acid (mtDNA) single nucleotide polymorphism (SNP) detection
    15. Analysis of deoxyribonucleic acid (DNA) sequence data using bioedit
    16. Ribonucleic acid (RNA) extraction
    17. Y-STR PCR amplification
    18. Human genetic analysis: paternity or missing persons cases and statistics
    19. Low copy number stochastic results
    20. Using in silico methods to construct a short tandem repeat (STR) deoxyribonucleic acid (DNA) sequence for cloning
    21. Deoxyribonucleic acid (DNA) extraction from botanical material and polymerase chain reaction (PCR) amplification
    22. Social, ethical and regulatory concerns
    23. Selected forensic DNA biology case studies
    Appendix:  Allele frequencies of CODIS loci

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