Fluorescence Spectroscopy

Edited by

  • Ludwig Brand, McCollum-Pratt Institute, Johns Hopkins University, Baltimore, MD, USA
  • Michael Johnson, University of Virginia Health Sciences Center, Charlottesville, USA

Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics.
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Biochemists, biophysicists, cell biologists, and molecular biologists.


Book information

  • Published: December 2008
  • ISBN: 978-0-12-374586-6

Table of Contents

Direct quantitation of Mg2+ - RNA interactions use of a fluorescent dyeDan Grilley, Ana Maria Soto, and David E. Draper A Method in Enzymology for Measuring Intracellular Protease Activities in Live Cells Beverly Z. Packard and Akira Komoriya Using Fluorophore-labeled Oligonucleotides to Measure Affinities of Protein-DNA InteractionsBrian J. Anderson and Joel F. Schildbach Site-Selective Red-Edge EffectsAlexander P. Demchenko Multiparametric Probing of Microenvironment with Solvatochromic Fluorescent Dyes Andrey S. Klymchenko and Alexander Demchenko Pteridine Probes for Nucleic Acid AnalysisMary E. Hawkins Application of Single Molecule Spectroscopy in Studying Enzyme Kinetics and Mechanism Jue Shi, Ari Gafni, and Duncan Steel Fluorescence Approaches in Quantifying Bio-Molecular InteractionsCatherine A. Royer and Suzanne Applications of Up-Conversion Methods in Studies of Proteins and PeptidesJay Knutson Spectroscopic Studies of Molecular Solvent RelaxationMark MaroncelliSingle Molecule Fluorescence Studies of the Dynamics of Gene RegulationJie XiaoNew Fluorescence Methods for Studies of Muscle ProteinsRobert E. DaleFluorescence Probes for Analysis of Membrane ProteinsAmitabha ChattopadhyayFluorescence Methods for Protein-Protein interaction in MembranesKalina HristovaFluorescence Detection of Specific IonsBernard Valeur Measuring Picomolar Intracellular Exchangable Zn in PC-12 Cells using a Ratiometric Fluorescence Biosensor R.A. Bozym Monitoring Free Zinc in the Mitochondrion Richard B Thompson, et al Metal-enhanced fluorescence: An emerging tool in biotechnologyChris D. Geddes, et alRadiative Decay Engineering, Surface Plasmon-Coupled Directional EmissionJoseph R Lakowicz, et alTime-resolved fluorescence anisotropy and the measurement of protein conformational flexibility David A. Johnson (University of California Riverside) Tranes and other new Analysis Procedures in wavelength resolved fluorescence.Pariasamy (Mumbay)From Quantum Theory to Molecular Fluorescence.D. ToptyginProbing Protein Electrostatics with Synthetic amino acids.Bruce Cohen (UCSF)Analysis of Complex Fluorescence Anisotropy DecayWilliam Laws, MontanaStudies of Segmental Protein Dynamics with Time and Energy ? Resolved Fluorescence SpectroscopyD. Toptygin and L. BrandComplex Fluorescence Decay of Trytophan in Proteins.Mary D. Barkley