Fluorescence Microscopy book cover

Fluorescence Microscopy

Super-Resolution and other Novel Techniques

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers.

With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research.

Researchers in cell biology, molecular biology, biochemistry, pathology and across biomedicine.

Hardbound, 260 Pages

Published: March 2014

Imprint: Academic Press

ISBN: 978-0-12-409513-7


  • "...a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science."--Anticancer Research, January 2015


  • Preface
    1: Supercritical excitation and emission near surfaces
    2: Adaptive optics for fluorescence microscopy of thick tissue
    3: Rapid polarization of fluorescence measurements in ex-vivo tissue
    4: Microscopy and nanoscale membrane organization
    5: Imaging entire organs
    6: RNA mimics of GFP
    7: High content/throughput imaging and cytometry via fluorescence and surface enhanced Raman scattering (SERS) microscopy
    8: Light sheet microscopy applications
    9: Cryo-ET-fluorescence super-resolution
    10: STED applications
    11: Use of Engeered nanoparticles based on fluorescence methods for live cell phonomena
    12: Illumination pattern correction
    13: Confocal stereology
    14: Multi-photon microscopy applications in biology
    15: TIRF 
    16: SIM
    17: The role of data analysis algorithms in super-resolution localization microscopy


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