Fed-Batch Fermentation book cover

Fed-Batch Fermentation

A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli

Fed-batch fermentation is primarily a practical guide for recombinant protein production in E. coli using a fed-batch fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - fed-batch fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.

Hardbound, 270 Pages

Published: October 2014

Imprint: Woodhead Publishing

ISBN: 978-1-907568-92-3

Contents

  • Introduction to fermentation; Typical recombinant E. coli host cells and plasmids used; Generation of recombinant clone and working cell bank; Generation of inoculants for fed-batch culture; Fed-batch culture method; Analysis of growth and production data; Parametric analysis for periplasmic soluble production of recombinant product.

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