Edited by

  • Fred Rainey, College of Basic Sciences, Louisiana State University, Baton Rouge, U.S.A.
  • Aharon Oren, Department of Plant and Environmental Sciences, The Institute of Life Sciences, The Hebrew University of Jerusalem, Israel

This latest volume in the Methods in Microbiology series provides an overview of the methods used for the isolation, cultivation and handling of a wide variety of extremophiles, both at the stage of their isolation from natural samples and in pure culture. It contains protocols specific for work with extremophiles, as well as adaptations of “standard” microbiology protocols modified to enable the handling of extremophiles. Emphasis will be on detailed time-tested protocols that are specific for work with extremophiles and adaptations of “standard” microbiology protocols that have to be modified to be suitable for handling extremophiles.
View full description


General microbiologists, industrial microbiologists, biotechnologists, molecular biologists, microbial ecologists, envirnomental and marine microbiologists


Book information

  • Published: May 2006
  • ISBN: 978-0-12-521536-7

Table of Contents

THERMOPHILESIn-situ activity studies in thermal environmentsThe isolation of thermophiles from deep-sea hydrothermal environmentsGrowth of hyperthermophilic microorganisms for physiological and nutritional studies Toward the large scale cultivation of hyperthermophiles at high temperature and high-pressureThe chemotaxonomic analysis of lipids from extremophilic bacteriaMembranes of thermophiles and other extremophilesCharacterization and quantification of compatible solutes in (hyper)thermophilic microorganismsFunctional genomics of the thermo-acidophilic archaeon Sulfolobus solfataricusHeat shock proteins in hyperthermophilesDeep-sea Thermococcales and their genetic elements: plasmids and virusesGenetic systems for Thermus Gene transfer systems for obligately anaerobic thermophilic bacteriaHyperthermophilic virus-host systems: detection and isolation Preservation of thermophilic microorganismsPSYCHROPHILESHandling psychrophilic microorganisms Proteins from psychrophilesALKALIPHILESCultivation of aerobic alkaliphiles Isolation, cultivation, and characterization of alkalithermophilesACIDOPHILESThe isolation and study of acidophilic microorganismsHALOPHILESCharacterization of natural communities of halophilic microorganismsCultivation of haloarchaeaHalophile extraction from ancient crystalsThe assessment of the viability of halophilic microorganisms in natural communitiesCharacterization of lipids of halophilic ArchaeaCharacterization of organic compatible solutes of halotolerant and halophilic organismsGenetic systems for halophilic archaea The isolation ?of viruses of halophilic microorganismsDetection, quantification and purification of halocins: peptide antibiotics from haloarchaeal extremophilesThe storage of halophilic bacteriaBAROPHILES Handling piezophilic microorganisms RADIATION-RESISTANT MICROORGANISMSMeasuring survival in microbial populations following exposure to ionizing radiationSTRICT ANAEROBESStrictly anaerobic microorganismsAPPLICATIONS OF EXTREMOPHILESIndustrial screening of extemophiles for valuable biomolecules