Essentials in Modern HPLC Separations

By
  • Serban Moldoveanu, RJ Reynolds Tobacco Co., Winston-Salem, NC, USA
  • Victor David, University of Bucharest, Faculty of Chemistry, Bucharest, Romania

This book discusses in a systematic manner the role of separation in HPLC, the types and characteristics of stationary phases and of mobile phases used in this technique, as well as other factors influencing the separation. The selection process of stationary and mobile phase for a specific separation is described as related to the physico-chemical characteristics of the molecules to be separated and of their matrix. All these subjects are discussed from the point of view of the new developments in HPLC. The book also includes a part presenting the practice of modern HPLC as necessary for applications, particularly related to the analysis of pharmaceutical and biological samples, food and beverages, environmental samples, etc.

Audience
Analytical chemists in industry, research, and universities

Hardbound, 550 Pages

Published: September 2012

Imprint: Elsevier

ISBN: 978-0-12-385013-3

Contents

  • 1 Basic Information About HPLC Separation
    2 Parameters that Characterize HPLC Analysis
    3 Equilibrium Types in HPLC
    4 Intermolecular Forces
    5 Retention Mechanisms in Different HPLC Types
    6 Stationary Phases and their Performance
    7 Mobile Phases and their Properties
    8 Solutes in HPLC
    9 HPLC Analysis

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