Essential Ion Channel Methods book cover

Essential Ion Channel Methods

The rapid growth of interest and research activity in ion channels is indicative of their fundamental importance in the maintenance of the living state. This volume was prepared with a view toward providing a sampling of the range of molecular and physical methods that are significant for the study of ion channels.

As part of the Reliable Lab Solutions series, Essential Ion Channel Methods brings together chapters from volumes 293 and 294 of Methods in Enzymology. The chapters have been selected by the editor and updated, when possible, by their original authors to include new research and references. The result is a set of chapters which make use of graphics, comparisons to other methods, and provide tricks and approaches that make it possible to adapt methods to other systems. Methods are presented in a fashion that allows their replication by individuals new to the field, yet providing valuable information for seasoned investigators.

Researchers in and students of biochemistry, molecular and cell biology, developmental biology, and genetics.

Paperback, 632 Pages

Published: June 2010

Imprint: Academic Press

ISBN: 978-0-12-382204-8


  • PART I - Assembly
    1. Assembly of Ion Channels
    ZuFang Sheng and Carol Deutsch

    PART II - Genetics
    2. Identification of Ion Channel-Associated Proteins Using the Yeast Two-Hybrid System
    Martin Niethammer and Morgan Sheng

    PART III - Electrophysiology
    3. Patch Clamp Studies of the Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel
    John W. Hanrahan, Zie Kone , Ceri J. Mathews,Jiexin Luo, Yanlin Jia  and Paul Lindsell

    4. Tight-Seal Whole-cell Patch Clamping of C. elegans Neurons
    S.R. Lockery and M.B. Goodman

    5. Gating Currents
    Enrico Stefani and Francisco Bezanilla

    6. Determining Ion Channel Permeation Properties
    Ted Begenisich

    PART IV - Expression Systems
    7. Expression of Ligand-Gated Ion Channels Using Using Semliki Forest Virus and Baculovirus
    Kathryn Radford and Gary Buell

    8. Recombinant Adenovirus-Mediated Expression in Nervous System of Genes Coding for Ion Channels and Other Molecules Involved in Synaptic Function
    Markus U. Ehrengruber, Markus Lanzrein, Youfeng Xu, Mark C. Jasek, David B. Kantor, Erin M. Schuman, Henry A. Lester, and Norman Davidson

    9. Transient Expression of Heteromeric Ion Channels
    Alison L. Eertmoed, Yolanda F. Vallejo, and William N. Green

    PART V - Model Simulations
    10. Computer Simulations and Modeling of Ion Channels
    Michael E. Green

    PART VI.  Physical
    11. Fluorescence Techniques for Measuring Ion Channel Activity
    Gönül Velicelebi, Kenneth A. Stauderman, Mark A. Varney, Michael Akong, Stephen D. Hess, and Edwin C. Johnson

    12. Ligand Binding Methods for Analysis of Ion Channel Structure and Function
    Steen E. Pedersen, Monica M. Lurtz, and Rao V. L. Papineni

    13. Three-dimensional structure of membrane proteins determined by two-dimensional crystallization, electron cryomicroscopy, and image analysis
    Mark Yeager, Vinzenz M. Unger, Alok K. Mitra

    14. Voltage Clamp Biosensors for Capillary Electrophoresis
    Owe Orwar, Kent Jardemark, Cecilia Farre, Ingemar Jacobson, Alexander Moscho, Jason B. Shear, Harvey A. Fishman, Sheri J. Lillard, Richard N. Zare

    15. Ion Channels As Tools to Monitor Lipid Bilayer-Membrane Protein Interactions: Gramicidin Channels As Molecular Force
    O. S. Andersen, C. Nielsen, A. M. Maer, J. A. Lundbæk, M. Goulian, and R. E. Koeppe I

    PART VII. Purification and Reconstitution
    16. Purification and Reconstitution of the Epithelial Chloride Channel Cystic Fibrosis Transmembrane Conductance Regulator
    Mohabir Ramjeesingh, Elizabeth Garani, Kevin Galley, Canhui Li, Yanchun Wang, Christine E. Bear

    17. Reconstitution of Native and Cloned Channels into Planar Bilayers
    I. Favre, Y.-M. Sun, and E. Moczydlowski

    PART VIII. Second Messengers and Biochemical Approaches
    18. Protein Phosphorylation of Ligand-Gated Ion Channels
    Andrew l. Mammen, Sunjeev Kamboj, and Richard L. Huganir

    19. Analysis of Ion Channel Associated Proteins
    Michael Wyszynski and Morgan Sheng

    20. Secondary Messenger Regulation of Ion Channels/Plant Patch Clamping
    Sarah M. Assmann and Lisa Romano

    PART IX. Special Channels
    21. ATP-Sensitive Potassium Channels
    Joseph Bryan, M. Schwanstecher, C. Schwanstecher, F. Chudziak, U. Panten, J.P. Clement IV, G. Gonzalez, and L. Aguilar-Bryan

    22. Simplified Fast Pressure-Clamp Technique for Studying Mechanically-Gated Channels
    Don W. McBride, Jr. and Owen P. Hamill

    23. Purification and Heterologous Expression of Inhibitory Glycine Receptors
    Bodo Laube and Heinrich Betz

    24. Functional Analyses of Aquaporin Water Channel Proteins
    Peter Agre, John C. Mathai, Barbara L. Smith, and Gregory M. Preston

    PART X. Toxins and Other Membrane Active Compounds
    25. Conus Peptides as Probes for Ion Channels
    J. Michael McIntosh, Baldomero M. Olivera, and Lourdes J. Cruz

    26. Scorpion Toxins as Tools for Studying Potassium Channels
    Maria Garcia, Markus Hanner, Hans-Günther Knaus, Robert Slaughter, Gregory J. Kaczorowski

    27. The Use of Planar Lipid Bilayer Membranes for Rapid Screening of Membrane Active Compounds
    Bruce L. Kagan, Tajb A. Mirzabekov, Antoly Y. Silberstein

    28. Antibodies to Ion Channels
    Angela Vincent, Ian Hart, Ashwin Pinto, and F. Anne Stephenson


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