Enzyme Kinetics and Mechanism, Part F: Detection and Characterization of Enzyme Reaction Intermediates
Methods in EnzymologyBy
- Daniel Purich, University of Florida, Gainesville, U.S.A.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.
Biochemists, biophysicists, pharmacologists, molecular biologists, analytical, organic, and medicinal chemists, and graduate students in these disciplines
Methods in Enzymology
Hardbound, 650 Pages
Published: October 2002
Imprint: Academic Press
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- Covalent Enzyme-Substrate Compounds: Detection and Catalytic CompetenceRapid Mix-Quench MALDI-TOF Mass Spectrometry for Analysis of Enzymatic SystemsPre-Steady-State Kinetics of Enzymatic Reactions Studied by Electrospray Mass Spectrometry with On-Line Rapid-Mixing TechniquesTrapping of -Glycosidase IntermediatesTrapping Covalent Intermediates on ß-Glycosidases2-Hydroxy-6-keto-nona-2,4-diene 1,9-Dioic Acid 5,6-Hydrolase: Evidence from18O Isotope Exchange for gem-Diol IntermediateNucleoside-Diphosphate Kinase: Structural and Kinetic Analysis of Reaction Pathway and Phosphohistidine IntermediateGalactose-1-Phosphate Uridylyltransferase: Kinetics of Formation and Reaction of Uridylyl-Enzyme Intermediate in Wild-Type and Specifically Mutated UridylyltransferasesKinetic Evidence for Covalent Phosphoryl-Enzyme Intermediate in Phosphotransferase Activity of Human Red Cell Pyrimidine NucleotidasesCharacterization of (2->6) -Sialyltransferase Reaction Intermediates: Use of Alternative Substrates to Unmask Kinetic Isotope EffectsUse of Sodium Borohydride to Detect Acyl-Phosphate Linkages in Enzyme ReactionsEvidence for Phosphotransferases Phosphorylated on Aspartate Residue in N-Terminal DXDX(T/V) MotifMurC and MurD Synthetases of Peptidoglycan Biosynthesis: Borohydride Trapping of Acyl-Phosphate IntermediatesTransaldolase B: Borohydride Reduction Trapping of Schiff Base Intermediate between Dihydroxyacetone and -Amino Group of Active-Site Lysine ResidueUse of NMR and Borohydride Trapping to Provide Evidence for Covalent Enzyme-Substrate Imine IntermediateDetection of Covalent Tetrahedral Adducts by Differential Isotope Shift ¹³C NMR: Acetyl-Enzyme Reaction Intermediate Formed by 3-Hydroxy-3-methylglutaryl-CoA SynthaseDetection of Intermediates in Reactions Catalyzed by PLP-Dependent Enzymes: O-Acetylserine Sulfhydrylase and Serine-Glyoxalate AminotransferaseProtein Tyrosine Phosphatase: X-Ray Crystallographic Observation of Cysteinyl-Phosphate Reaction IntermediateGTP:GTP Guanylyltransferase: Trapping Procedures for Detecting and Characterizing Chemical Nature of Enzyme-Nucleotide Phosphoramidate Reaction Intermediate-Glutamyl Thioester Intermediate in Glutaminase Reaction Catalyzed by Escherichia coli Asparagine Synthetase B-Glutamyltranspeptidase and -Glutamyl Peptide Ligases: Fluorophosphonate and Phosphonodifluoromethyl Ketone Analogs as Probes of Tetrahedral Transition State and -Glutamyl-Phosphate IntermediateStoichiometric Redox Titrations of Complex MetalloenzymesUrate Oxidase: Single-Turnover Stopped-Flow Techniques for Detecting Two Discrete Enzyme-Bound IntermediatesNitric Oxide Synthase: Use of Stopped-Flow Spectroscopy and Rapid-Quench Methods in Single-Turnover Conditions to Examine Formation and Reactions of Heme- O2 Intermediate in Early CatalysisMyeloperoxidase: Kinetic Evidence for Formation of Enzyme-Bound Chlorinating IntermediateTime-Resolved Resonance Raman Spectroscopy of Intermediates in Cytochrome OxidasePorphobilinogen Deaminase: Accumulation and Detection of Tetrapyrrole Intermediates Using Enzyme ImmobilizationAdenosylcobalamin-Dependent Glutamate Mutase: Pre-Steady-State Kinetic Methods for Investigating Reaction MechanismRibonucleotide Reductase: Kinetic Methods for Demonstrating Radical Transfer Pathway in Protein R2 of Mouse Enzyme in Generation of Tyrosyl Free RadicalGalactose Oxidase: Probing Radical Mechanism with Ultrafast Radical ProbeKinetic Characterization of Transient Free Radical Intermediates in Reaction of Lysine 2,3-Aminomutase by EPR Lineshape AnalysisDemonstration of Peroxodiferric Intermediate in M-Ferritin Ferroxidase Reaction Using Rapid Freeze-Quench Mössbauer, Resonance Raman, and XAS SpectroscopiesA Survey of Covalent, Ionic, and RadicalIntermediates in Enzyme-Catalyzed Reactions