Digital Microscopy

Edited by

  • Greenfield Sluder, University of Massachusetts Medical Center, Shrewsbury, U.S.A.
  • David Wolf, University of Massachusetts Medical Center, Shrewsbury, U.S.A.

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.
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Audience

Cell and molecular biologists and researchers utilizing digitial microscopy techniques.

 

Book information

  • Published: August 2013
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-407761-4


Table of Contents

  1. Microscope Basics
    Greenfield Sluder and Joshua J. Nordberg
  2. The Optics of Microscope Image Formation
    David E. Wolf
  3. Proper Alignment of the Microscope
    Rudi Rottenfusser
  4. Fundamentals of Fluorescence and Fluorescence Microscopy
    David E. Wolf
  5. Fluorescent Protein Applications in Microscopy
    Whitney L. Johnson and Aaron F. Straight
  6. Live-Cell Fluorescence Imaging
    Jennifer C. Waters
  7. Practical Aspects of Adjusting Digital Cameras
    Joshua J. Nordberg and Greenfield Sluder
  8. Cameras for Digital Microscopy
    Kenneth R. Spring
  9. A High-Resolution Multimode Digital Microscope System
    E. D. Salmon, Sidney L. Shaw, Jennifer C. Waters, Clare M. Waterman-Storer, Paul S. Maddox, Elaine Yeh, and Kerry Bloom
  10. Electronic Cameras for Low-Light Microscopy
    Ivan Rasnik, Todd French, Ken Jacobson and Keith Berland
  11. Camera Technologies for Low Light Imaging - Overview and Relative Advantages
    Butch Moomaw
  12. Post-Processing for Statistical Image Analysis in Light Microscopy
    Richard A. Cardullo and Edward H. Hinchcliffe
  13. 65,000 Shades of Grey: Use of Digital Image Files in Light Microscopy
    Sidney L. Shaw and Edward H. Hinchcliffe
  14. Quantitative Analysis of Digital Microscope Images
    David E.Wolf, Champika Samarasekera, and Jason R. Swedlow
  15. Evaluating Optical Aberrations Using Fluorescent Microspheres: Methods, Analysis, and Corrective Actions
    Paul C. Goodwin
  16. Ratio Imaging: Practical Considerations for Measuring Intracellular Ca2þ and pH in Living Cells
    Nathan O’Connor and Randi B. Silver
  17. Quantitative Fluorescence Microscopy and Image Deconvolution
    Jason R. Swedlow
  18. Practical Aspects of Quantitative Confocal Microscopy
    John M. Murray
  19. Theoretical Principles and Practical Considerations for Fluorescence Resonance Energy Transfer Microscopy
    Richard A. Cardullo
  20. Fluorescence Lifetime Imaging Microscopy for Quantitative Biological Imaging
    L.-C. Chen, W.R. Lloyd III, C.-W. Chang, D. Sud, and M.-A. Mycek
  21. Fluorescence Correlation Spectroscopy: Molecular Complexing in Solution and in Living Cells
    Dylan A. Bulseco and David E. Wolf
  22. Breaking the Resolution Limit in Light Microscopy
    Rainer Heintzmann and Gabriella Ficz
  23. Circumventing Photodamage in Live-Cell Microscopy
    Valentin Magidson and Alexey Khodjakov
  24. A User’s Guide to Localization-Based Super-Resolution Fluorescence Imaging
    Graham T. Dempsey
  25. Ratiometric Imaging of FRET-Biosensors for the Rho-Family GTPases
    Désirée Spiering, Jose Javier Bravo-Cordero, Yasmin Moshfegh, Veronika Miskolci and Louis Hodgson
  26. Tip-Enhanced Raman Spectroscopy for the base interrogation of DNA
    Noah Kolodziejski