Determining Mycotoxins and Mycotoxigenic Fungi in Food and Feed
- Sarah De Saeger, Ghent University, Belgium
Mycotoxins - toxic secondary metabolites produced by mycotoxigenic fungi - pose a significant risk to the food chain. Indeed, they may be the most hazardous of all food contaminants in terms of chronic toxicity and legislative limits on their levels in food and feed continue to be developed worldwide. Rapid and reliable methods for the determination of both mycotoxigenic fungi and mycotoxins in food and feed are therefore essential. This book reviews current and emerging methods in this area.View full description
Part one focuses on the essentials of mycotoxin determination, covering sampling, sample preparation and clean-up and key determination techniques, such as chromatographic separation, liquid chromatography-mass spectrometry and immunochemical methods. Part two then goes on to describe quality assurance, official methods and performance criteria for determining mycotoxins in food and feed. Topics covered include laboratory accreditation, method validation and measurement uncertainty. The development and analysis of biomarkers for mycotoxins are discussed in part three. Individual chapters focus on detecting exposure in humans and animals. Part four is concerned with the processes involved in determining mycotoxigenic fungi in food and feed. It also describes the identification of genes and gene clusters involved in mycotoxin synthesis, as well as DNA barcoding of toxigenic fungi. Finally, part five explores some of the emerging methods for mycotoxin analysis, ranging from bio-sensing to spectroscopic techniques.
With its distinguished editor and international team of contributors, Determining mycotoxins and mycotoxigenic fungi in food and feed is a standard reference for all those concerned with reducing mycotoxin contamination in the food chain.
All those concerned with reducing mycotoxin contamination in the food chain
- Published: February 2011
- Imprint: Woodhead Publishing
- ISBN: 978-1-84569-674-0
Table of ContentsPart 1 Determining mycotoxins in food and feed: Sampling strategies to control mycotoxins; Sample preparation and clean up for mycotoxin analysis: Principles, applications and recent developments; Chromatographic separation techniques for determination of mycotoxins in food and feed; Mass spectrometry in multi-mycotoxin and fungal spore analysis; Immunochemical methods for rapid mycotoxin detection in food and feed. Part 2 Quality Assurance and official methods for determining mycotoxins in food and feed: Official methods and performance criteria for determining mycotoxins in food and feed; Ensuring the quality of results from food control laboratories: Laboratory accreditation, method validation and measurement uncertainty. Part 3 Development and analysis of biomarkers for mycotoxins: Developing biomarkers of human exposure to mycotoxins; Developing mechanism-based and exposure biomarkers for mycotoxins in animals. Part 4 Determining mycotoxigenic fungi in food and feed: Rationale for a polyphasic approach in the identification of mycotoxigenic fungi; Molecular identification of mycotoxigenic fungi in food and feedstuffs; Identification of genes and gene clusters involved in mycotoxin synthesis; DNA barcoding of mycotoxigenic fungi: A perspective. Part 5 Emerging methods for mycotoxin analysis in food and feed: Emerging bio-sensing methods for mycotoxin analysis; Masked mycotoxins in food and feed: Challenges and analytical approaches; Spectroscopic techniques for fungi and mycotoxin detection.