Cytochemical Staining Methods for Electron Microscopy, 14

Edited By

  • P.R. Lewis, Physiology Laboratory, University of Cambridge, Cambridge, UK
  • D.P. Knight, King Alfred's College, Winchester, UK

The series Practical Methods in Electron Microscopy, edited by Audrey M. Glauert has an international reputation asa unique source of practical information for all electron microscopists.Each book of the series starts from first principles, assuming nospecialist knowledge, and is complete in itself. The series willeventually cover the whole range of techniques for electron microscopy.

In this latest volume in the popular series, Peter Lewis and David Knight describe in practical detail the whole range of staining techniques for electron microscopy, including contrast staining for ultrastructural studies, specific cytological staining methods, and enzyme cytochemical techniques.

The chemical basis of cytochemical methods is discussed and analysed in a straightforward manner with great clarity. In consequence this book will help the beginner to choose between the sometimes bewildering variety of published technical procedures.

Electron microscopists will find this volume to be an invaluable and compact source-book for all those cytochemical techniques that have proved to be both reliable and convenient to use. As with previous titles in this popular series, the unique type of practical guidance provided will be of value for many years to come.

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Book information

  • Published: August 1992
  • Imprint: ELSEVIER
  • ISBN: 978-0-444-89387-1


...another outstanding volume in...truly informative and interesting examples...explicitly written chapters are well illustrated...highly recommended as an essential reference work for any EM laboratory.

When the first edition was published in 1977 it became essential reading, to new and existing users; this updated second editionis equally essential.
Microscopy and Analysis

Table of Contents

Chapter 1. Introduction (P.R. Lewis and D.P. Knight). 1.1. The Objectives ofSection Staining. 1.2. Contrast in Transmission Electron Microscopy. 1.3.Fixation of tissues. 1.4. Preparation of Tissues. 1.5. Special EmbeddingTechniques. 1.6. Safety in the Laboratory. Chapter 2. Cytological StainingMethods (D.P. Knight and P.R. Lewis). 2.1. Resin Sections for LightMicroscopy. 2.2. Procedures for Handling Ultrathin Sections. 2.3. LeadStains. 2.4. Uranyl Acetate. 2.5. A Procedure for Double Staining. 2.6.Osmium Tetroxide. 2.7. Other Cytological Stains. 2.8. Tracers in ElectronMicroscopy. Chapter 3. General Cytochemical Methods (D.P. Knight and P.R.Lewis). 3.1. The limitations of present techniques. 3.2. CytochemicalMethods with Broad Specificities. 3.3. Methods for Nucleic Acids. 3.4.General Methods for Proteins. 3.5. Specific Methods for Carbohydrates. 3.6.Methods for Lipids. 3.7. Precipitation Methods for Inorganic Ions. 3.8.Extractive Methods. Chapter 4. Metal Precipitation Methods for HydrolyticEnzymes (P.R. Lewis). 4.1. The Principles of Enzyme Cytochemistry. 4.2.Some Practical Problems of Enzymes Cytochemistry. 4.3. Methods for AcidHydrolases and Related Techniques. 4.4. Enzymes Which Hydrolyze ATP. 4.5.Other Phosphatases. 4.6. General Techniques for Esterases. 4.7. TechniquesSpecific for the Cholinesterases. Chapter 5. Other Cytochemical Methods forEnzymes (P.R. Lewis). 5.1. Histochemistry of the Oxidases. 5.2. ABiochemical Background to Dehydrogenase Histochemistry. 5.3. Ferricyanideas a Histochemical Reagent. 5.4. The Use of Tetrazolium Salts. 5.5. Azo-dye Coupling Techniques. 5.6 Methods for Other Enzymes. 5.7. RecentAdvances and Future Development in Cytochemistry. Appendix 1. Stock solutions. Appendix 2. List of suppliers.