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Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention, Volume 1
1st Edition - July 30, 2014
Editors: Charles Rupprecht, Thirumeni Nagarajan
Language: English
Hardback ISBN:9780128000144
9 7 8 - 0 - 1 2 - 8 0 0 0 1 4 - 4
eBook ISBN:9780128004654
9 7 8 - 0 - 1 2 - 8 0 0 4 6 5 - 4
Laboratory Techniques in Rabies Diagnosis, Research and Prevention provides a basic understanding of the current trends in rabies. It establishes a new facility for rabies su…Read more
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Laboratory Techniques in Rabies Diagnosis, Research and Prevention provides a basic understanding of the current trends in rabies. It establishes a new facility for rabies surveillance, vaccine and antibody manufacturing. It offers clarity about the choice of laboratory methods for diagnosis and virus typing, of systems for producing monoclonal and polyclonal antibodies and of methods for testing potency of vaccines and antibodies.
The book covers advancements in the classical methods described as well as recent methods and approaches pertaining to rabies diagnosis and research.
Supplies techniques pertaining to rabies diagnosis and research
Provides an update on the conventional and modern vaccines for rabies prevention
Offers updates on the full length antibodies and antibody fragments for post exposure prophylaxis of rabies
Presents technique descriptions that can be used to be compared to industry protocols to identify and establish potential new techniques
Students, researchers, health professionals, biologists in microbiology, industry personnel and laboratory personal in the vaccine, biopharmaceutical and diagnostic industries
Foreword
List of Contributors
Part One: Introduction
Chapter One. Basic Facts about Lyssaviruses
1.1 Introduction
1.2 Virion and Genome Organization
1.3 Phylogeny and Serologic Cross-Reactivity of Lyssaviruses
1.4 Host Range
1.5 Pathobiology
References
Part Two: Rabies Diagnosis
Section A: Demonstration of Viral Subunits and Antigens
Chapter Two. Demonstration of Lyssavirus Antigens by a Direct Rapid Immunohistochemical Test
2.1 Introduction
2.2 Materials
2.3 Methods
2.4 Discussion
References
Chapter Three. Demonstration of Rabies Virus Antigens by a Latex Agglutination Test
3.1 Introduction
3.2 Materials
3.3 Methods
3.4 Discussion
References
Chapter Four. Rabies Diagnosis: Demonstration of Viral Antigens by Flow Cytometry
4.1 Introduction
4.2 Materials
4.3 Methods
4.4 Discussion
Acknowledgments
References
Chapter Five. Demonstration of Rabies Virus Antigens by an Immunochromatographic Strip Test
5.1 Introduction
5.2 Materials
5.3 Methods
5.4 Discussion
References
Section B: Demonstration of Viral Nucleic Acids
Chapter Six. Demonstration of African Lyssavirus RNA with Real-Time Polymerase Chain Reaction
6.1 Introduction
6.2 Materials
6.3 Methods
6.4 Discussion
References
Chapter Seven. Real-Time Quantitative Polymerase Chain Reaction for the Demonstration of Lyssavirus Nucleic Acid
7.1 Real-Time Quantitative Polymerase Chain Reaction for the Demonstration of Lyssavirus Nucleic Acid
7.2 Methodology
7.3 Discussion
Acknowledgments
References
Chapter Eight. Reverse Transcription-Loop-Mediated Isothermal Amplification System for the Detection of Rabies Virus
8.1 Introduction
8.2 Materials
8.3 Methods
8.4 Discussion
Acknowledgments
References
Chapter Nine. Detection of Viral Nucleic Acids by In Situ Hybridization
9.1 Introduction
9.2 Materials
9.3 Methods
9.4 Discussion
Acknowledgments
References
Chapter Ten. Genetic Characterization via Pyrosequencing
10.1 Introduction
10.2 Materials
10.3 Methods
10.4 Discussion
Acknowledgments
References
Section C: Demonstration of Viral Antibodies and Immune Complexes
Chapter Eleven. Demonstration of Immune Complexes by Capture Enzyme-Linked Immunosorbent Assay
11.1 Introduction
11.2 Materials
11.3 Methods
11.4 Discussion
Acknowledgments
References
Chapter Twelve. Demonstration of Viral Antibodies by an Immunochromatographic Strip Test
12.1 Introduction
12.2 Materials
12.3 Methods
12.4 Discussion
Acknowledgments
References
Chapter Thirteen. Rabies Diagnosis: Demonstration of Viral Antibodies by Flow Cytometry
13.1 Introduction
13.2 Materials
13.3 Methods
13.4 Discussion
Acknowledgments
References
Chapter Fourteen. Demonstration of Rabies Antibody by a Latex Agglutination Test
14.1 Introduction
14.2 Materials
14.3 Methods
14.4 Discussion
References
Chapter Fifteen. Demonstration of Viral Antibodies by Pseudotype Virus Neutralization
15.1 Introduction
15.2 Materials
15.3 Methods
15.4 Discussion
Acknowledgments
References
Section D: Typing/Differentiation of Lyssaviruses
Chapter Sixteen. Sanger Sequencing of Lyssaviruses
16.1 Background
16.2 Methodology
16.3 Discussion
Acknowledgments
References
Chapter Seventeen. Next Generation Sequencing of Lyssaviruses
17.1 Background
17.2 Methodology
17.3 Discussion
Acknowledgments
References
Chapter Eighteen. Genetic Characterization of Rabies Viruses by In Situ Hybridization
18.1 Introduction
18.2 Materials
18.3 Methods
18.4 Discussion
Acknowledgments
References
Chapter Nineteen. Oligonucleotide Microarray: Applications for Lyssavirus Speciation
19.1 Introduction
19.2 Methodology
19.3 Discussion
Acknowledgments
References
Chapter Twenty. Demonstration of Lyssavirus Nucleic Acids by Pyrosequencing
20.1 Introduction
20.2 Materials
20.3 Methods
20.4 Discussion
Acknowledgments
References
Section E: Demonstration of Viral-Induced Changes in Tissues/Organs
Chapter Twenty One. Rabies Diagnosis: MR Imaging
21.1 Introduction
21.2 Materials
21.3 Methods
21.4 Discussion
Acknowledgments
References
Part Three: Rabies Biologics
Section F: Rabies Vaccines for Humans or Other Animals
Chapter Twenty Two. Attenuated Vaccines for Veterinary Use
22.1 Introduction
22.2 Materials
22.3 Methods
22.4 Discussion
References
Chapter Twenty Three. Pox Viral Vectored Vaccines for Rabies
Chapter Twenty Five. Adenoviral Vectors as Vaccine Carriers for Prevention of Rabies
25.1 Introduction
25.2 Basic Characteristics of Ad Viruses
25.3 Types of Ad Vectors
25.4 Transgene Product for Rabies Vaccines
25.5 Construction of Ad Vectors
25.6 Ad Virus Rescue and Expansion
25.7 Quality Control of Ad Vectors
25.8 Discussion
References
Section G: Rabies Antibodies/Fragments
Chapter Twenty Six. Production of Human Monoclonal Antibodies using Mammalian Expression Systems
26.1 Introduction
26.2 Materials
26.3 Methods
Acknowledgments
References
Chapter Twenty Seven. Generation of Immune Globulin Single Variable Domains by Display Technologies
27.1 Introduction
27.2 Materials
27.3 Methods
27.4 Discussion
27.5 Experimental Tips
Acknowledgments
References
Part Four: Appendix
Appendix A
Appendix B
Appendix C. Filter Paper Technology for Sampling, Storage, and Shipment of Rabies Suspect Samples for the Identification of Viral Nucleic Acids
C.1 Introduction
C.2 Materials
C.3 Methods
C.4 Discussion
Acknowledgments
References
Index
No. of pages: 350
Language: English
Edition: 1
Published: July 30, 2014
Imprint: Academic Press
Hardback ISBN: 9780128000144
eBook ISBN: 9780128004654
CR
Charles Rupprecht
Professor Charles E. Rupprecht is the Director of Research at the Global Alliance for Rabies Control and is the previous Director of WHO Collaborating Center for Rabies Research. He has won numerous federal service awards including the National Center for Infectious Diseases Public Health Protection Research Award in 2005.
Affiliations and expertise
Global Alliance for Rabies Control
TN
Thirumeni Nagarajan
Professor Nagarajan Thirumeni is a professor in biotechnology and the manager of Indian Immunologics Ltd. as well as the general manager of research and development in the biologics division of E. Ltd, Hyderabad, India.
Affiliations and expertise
Biological E. Limited, Hyderabad, India
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