Cryo-EM, Part C book cover

Cryo-EM, Part C

Analyses, Interpretation, and Case Studies

This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

Audience

Biochemists, structural biologists, molecular biologists, cell biologists, and microbiologists

Included in series
Methods in Enzymology

Hardbound, 380 Pages

Published: October 2010

Imprint: Academic Press

ISBN: 978-0-12-384993-9

Contents

    1. Analyses of Subnanometer Resolution Cryo-EM Density Maps
    2. Matthew L. Baker, Mariah R. Baker Corey F. Hryc Frank DiMaio

    3. Methods for Segmentation and interpretation of cryo-EM data
    4. Niels Volkmann

    5. Integration of Cryo-EM with atomic and protein: protein interaction data
    6. Friedrich Forster

    7. Unified Data Resource for CryoEM
    8. Catherine L. Lawson

    9. Electron crystallography and aquaporins
    10. Thomas Walz, Andreas D. Schenk, Richard K. Hite, Andreas Engel, Yoshinori Fujiyoshi

    11. Cryo-Electron Microscopy Applications in the Study of Tubulin Structure, Microtubule Architecture, Dynamics and Assemblies, and Interaction of Microtubules with Motors
    12. Kenneth H. Downin and Eva Nogales

    13. Helical crystallization of two example membrane proteins: MsbA and the Ca2+-ATPase
    14. Howard S. Young, John Paul Glaves, Lauren Fisher, Andrew Ward

    15. Multi-particle cryo-EM of ribosomes
    16. Christian M.T. Spahn, Justus Loerke, Jan Giesebrecht

    17. Single-particle electron microscopy of animal fatty acid synthase: Describing macromolecular rearrangements that enable catalysis
    18. Francisco Asturias, Edward J. Brignole

    19. Tomography of actin cytoskeletal networks
    20. Dorit Hanein

    21. Visual Proteomics
    22. Friedrich Förster, Bong-Gyoon Han, and Martin Beck

    23. Cryo-Electron Tomography of Eukaryotic Cells
    24. Ohad Medalia, Asaf Mader , Nadav Elad

    25. 3d Visualization Of Hiv Virions By Cryo Electron Tomography
    26. Jun Liu, Elizabeth R. Wright, Hanspeter Winkler

    27. Automation in Single-Particle Electron Microscopy: Connecting the Pieces

    Bridget Carragher, Dmitry Lyumkis, Arne Moeller, Anchi Cheng, Amber Herold, Eric Hou, Christopher Irving, Erica L. Jacovetty, Pick-Wei Lau, Anke Mulder, James Pulokas, Joel D. Quispe, Neil R. Voss, Clinton S. Potter

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