Cryo-EM, Part C

Analyses, Interpretation, and Case Studies

  • Grant Jensen

This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
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Biochemists, structural biologists, molecular biologists, cell biologists, and microbiologists


Book information

  • Published: October 2010
  • ISBN: 978-0-12-384993-9

Table of Contents

  1. Analyses of Subnanometer Resolution Cryo-EM Density Maps
  2. Matthew L. Baker, Mariah R. Baker Corey F. Hryc Frank DiMaio

  3. Methods for Segmentation and interpretation of cryo-EM data
  4. Niels Volkmann

  5. Integration of Cryo-EM with atomic and protein: protein interaction data
  6. Friedrich Forster

  7. Unified Data Resource for CryoEM
  8. Catherine L. Lawson

  9. Electron crystallography and aquaporins
  10. Thomas Walz, Andreas D. Schenk, Richard K. Hite, Andreas Engel, Yoshinori Fujiyoshi

  11. Cryo-Electron Microscopy Applications in the Study of Tubulin Structure, Microtubule Architecture, Dynamics and Assemblies, and Interaction of Microtubules with Motors
  12. Kenneth H. Downin and Eva Nogales

  13. Helical crystallization of two example membrane proteins: MsbA and the Ca2+-ATPase
  14. Howard S. Young, John Paul Glaves, Lauren Fisher, Andrew Ward

  15. Multi-particle cryo-EM of ribosomes
  16. Christian M.T. Spahn, Justus Loerke, Jan Giesebrecht

  17. Single-particle electron microscopy of animal fatty acid synthase: Describing macromolecular rearrangements that enable catalysis
  18. Francisco Asturias, Edward J. Brignole

  19. Tomography of actin cytoskeletal networks
  20. Dorit Hanein

  21. Visual Proteomics
  22. Friedrich Förster, Bong-Gyoon Han, and Martin Beck

  23. Cryo-Electron Tomography of Eukaryotic Cells
  24. Ohad Medalia, Asaf Mader , Nadav Elad

  25. 3d Visualization Of Hiv Virions By Cryo Electron Tomography
  26. Jun Liu, Elizabeth R. Wright, Hanspeter Winkler

  27. Automation in Single-Particle Electron Microscopy: Connecting the Pieces

Bridget Carragher, Dmitry Lyumkis, Arne Moeller, Anchi Cheng, Amber Herold, Eric Hou, Christopher Irving, Erica L. Jacovetty, Pick-Wei Lau, Anke Mulder, James Pulokas, Joel D. Quispe, Neil R. Voss, Clinton S. Potter