Cryo-EM Part B: 3-D Reconstruction

  • Grant Jensen

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
View full description

Audience

Biochemists, structural biologists, molecular biologists, cell biologists, and microbiologists

 

Book information

  • Published: October 2010
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-384991-5


Table of Contents

  1. Fundamentals of three-dimensional reconstruction from projections
  2. Pawel A. Penczek

  3. Image Restoration in Cryo-electron Microscopy
  4. Pawel A. Penczek

  5. Resolution measures in molecular electron microscopy
  6. Pawel A. Penczek

  7. 3D reconstruction from 2D crystal image and diffraction data
  8. Andreas Schenk, Daniel Castaño-Díez, Bryant Gipson, Marcel Arheit, Xiangyan Zeng, and Henning Stahlberg

  9. Fourier-Bessel Reconstruction of Helical Assemblies
  10. David L Stokes, Ruben Diaz & William, J. Rice

  11. Reconstruction of Helical Filaments and Tubes
  12. Edward H Egelman

  13. Three-Dimensional Asymmetric Reconstruction of Tailed Bacteriophage
  14. Jinghua Tang, Robert S. Sinkovits, and Timothy S. Baker

  15. Single Particle Analysis at High Resolution
  16. Yao Cong and Steven J. Ludtke

  17. The Orthogonal Tilt Reconstruction method
  18. Andres Leschziner

  19. An introduction to maximum-likelihood methods in cryo-EM
  20. Fred J. Sigworth, Peter C. Doerschuk, Jose-Maria Carazo, and Sjors H.W. Scheres

  21. Classification of structural heterogeneity by maximum-likelihood methods
  22. Sjors H.W. Scheres

  23. Methods for three-dimensional reconstruction of heterogeneous assemblies
  24. Elena V Orlova, Helen R. Saibil

  25. Alignment of cryo-electron tomography datasets
  26. Fernando Amat, Daniel Casta˜no-Diez, Albert Lawrence, Farshid Moussavi, Hanspeter Winkler, & Mark Horowitz

  27. Correcting for the Ewald sphere in high resolution single particle reconstructions
  28. Grant J Jensen, Peter A. Leong, Xuekui Yu, & Z. Hong Zhou

  29. Software Tools For Molecular Microscopy: An Open-Text Wikibook

Bridget Carragher, Neil R. Voss, Clinton S. Potter, & Ross Smith