Cryo-EM Part B: 3-D Reconstruction book cover

Cryo-EM Part B: 3-D Reconstruction

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.


Biochemists, structural biologists, molecular biologists, cell biologists, and microbiologists

Included in series
Methods in Enzymology

Hardbound, 456 Pages

Published: October 2010

Imprint: Academic Press

ISBN: 978-0-12-384991-5


    1. Fundamentals of three-dimensional reconstruction from projections
    2. Pawel A. Penczek

    3. Image Restoration in Cryo-electron Microscopy
    4. Pawel A. Penczek

    5. Resolution measures in molecular electron microscopy
    6. Pawel A. Penczek

    7. 3D reconstruction from 2D crystal image and diffraction data
    8. Andreas Schenk, Daniel Castaño-Díez, Bryant Gipson, Marcel Arheit, Xiangyan Zeng, and Henning Stahlberg

    9. Fourier-Bessel Reconstruction of Helical Assemblies
    10. David L Stokes, Ruben Diaz & William, J. Rice

    11. Reconstruction of Helical Filaments and Tubes
    12. Edward H Egelman

    13. Three-Dimensional Asymmetric Reconstruction of Tailed Bacteriophage
    14. Jinghua Tang, Robert S. Sinkovits, and Timothy S. Baker

    15. Single Particle Analysis at High Resolution
    16. Yao Cong and Steven J. Ludtke

    17. The Orthogonal Tilt Reconstruction method
    18. Andres Leschziner

    19. An introduction to maximum-likelihood methods in cryo-EM
    20. Fred J. Sigworth, Peter C. Doerschuk, Jose-Maria Carazo, and Sjors H.W. Scheres

    21. Classification of structural heterogeneity by maximum-likelihood methods
    22. Sjors H.W. Scheres

    23. Methods for three-dimensional reconstruction of heterogeneous assemblies
    24. Elena V Orlova, Helen R. Saibil

    25. Alignment of cryo-electron tomography datasets
    26. Fernando Amat, Daniel Casta˜no-Diez, Albert Lawrence, Farshid Moussavi, Hanspeter Winkler, & Mark Horowitz

    27. Correcting for the Ewald sphere in high resolution single particle reconstructions
    28. Grant J Jensen, Peter A. Leong, Xuekui Yu, & Z. Hong Zhou

    29. Software Tools For Molecular Microscopy: An Open-Text Wikibook

    Bridget Carragher, Neil R. Voss, Clinton S. Potter, & Ross Smith


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