Cryo-EM Part A: Sample Preparation and Data Collection book cover

Cryo-EM Part A: Sample Preparation and Data Collection

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

Audience

Biochemists, structural biologists, molecular biologists, cell biologists, and microbiologists

Included in series
Methods in Enzymology

Hardbound, 464 Pages

Published: October 2010

Imprint: Academic Press

ISBN: 978-0-12-374906-2

Contents

  • Historical perspective

    3D reconstruction from electron micrographs: a personal account of its development

    David DeRosier

    1. Preparation of 2-D crystals
    2. Henning Stahlberg

    3. Helical Crystallization of Soluble and Membrane Binding Proteins
    4. Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie

    5. Plunge Freezing for Electron Cryomicroscopy
    6. Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen

    7. A Practical Guide to the Use of Monolayer Purification and Affinity Grids
    8. Thomas Walz, Deborah F. Kelly, Danijela Dukovski

    9. GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM
    10. Holger Stark

    11. Cryo-negative staining of macromolecular assemblies
    12. Sacha De Carlo, Holger Stark

    13. Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM
    14. Fred J. Sigworth, Liguo Wang

    15. Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing
    16. Mark S. Ladinsky

    17. Site-Specific Biomolecule Labeling with Gold Clusters
    18. Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld

    19. How to Operate a Cryo-Electron Microscope
    20. Huilin Li, Jingchuan Sun

    21. Collecting electron crystallographic data of two-dimensional protein crystals
    22. Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng

    23. Automated Data Collection for Electron Microscopic Tomography
    24. David Agard, Shawn Q. Zheng, J. W. Sedat

    25. Correlated Light and Electron Cryomicroscopy
    26. Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jürgen M. Plitzkod, Cindi L. Schwartz

    27. Phase Plates for Transmission Electron Microscopy
    28. Radostin Danev, Kuniaki Nagayama

    29. Radiation damage in electron cryomicroscopy

    John L. Rubinstein, Lindsay A. Baker

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