Comprehensive Biophysics


  • Edward Egelman, Editor-in-chief of Biophysical Journal. Elected fellow of the Biophysical Society and of the American Academy of Microbiology.

Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics will help bridge that communication gap.

Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge.

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Researchers, advanced undergraduate and graduate students, postdoctoral fellows, senior investigators, corporate customers (such as biotech companies) and libraries from Physics, Chemistry and Life Sciences departments.


Book information

  • Published: April 2012
  • ISBN: 978-0-12-374920-8

Table of Contents

Volume 1: Biophysical Techniques for Structural Characterization of Macromolecules (Jane Dyson, The Scripps Research Institute, CA, USA)

Volume 2: Biophysical Techniques for Characterization of Cells (Petra Schwille, TU Dresden, Dresden, Germany)

Volume 3: The Folding of Proteins and Nucleic Acids (Valerie Daggett, University of Washington, WA, USA)

Volume 4: Molecular Motors and Motility (Yale Goldman & E. Michael Ostap, University of Pennsylvania, PA, USA)

Volume 5: Membranes (Lukas Tamm, University of Virginia, VA, USA)

Volume 6: Channels (Mauricio Montal, University of California, San Diego, CA, USA)

Volume 7: Cell Biophysics (Denis Wirtz, Johns Hopkins University, Baltimore, MD, USA)

Volume 8: Bioenergetics (Stuart Ferguson, University of Oxford, UK)

Volume 9: Simulation and Modeling (Harel Weinstein, Weill Cornell Medical College, NY, USA)


Full list of contents:

Cantor and Schimmel - 30 Years Later
Efficient Strategies for Production of Eukaryotic Proteins
X-ray Crystallography: Crystallization
X-ray crystallography | Data Collection Strategies and Resources
X-ray crystallography | Phasing of X-ray Data
X-ray crystallography | Refinement of X-ray Crystal Structures
X-ray crystallography | Structure Validation and Analysis
NMR Spectroscopy | Solution methods
NMR Spectroscopy | Solid state NMR methods
NMR Spectroscopy | Membrane protein methods: The hybrid solution/solid-state NMR method
NMR Spectroscopy | Labeling techniques
NMR Spectroscopy | NMR Relaxation methods
Electron microscopy | 2D crystals
Electron microscopy | Cryo-Electron Microscopy and Tomography of Virus Particles
Mass spectrometry
Ultrafast structural dynamics of biological systems
Electron Magnetic Resonance
Computing structure, dynamics, and thermodynamics of proteins
Rapid Mixing Techniques for the Study of Enzyme Catalysis
Other spectroscopy - UV-Vis, CD, Raman, vibrational CD applied in biophysical research
Fluorescence and FRET: Theoretical Concepts 101
Elucidating cellular structures | Confocal laser scanning microscopy
Elucidating cellular structures | Two-photon microscopy
Elucidating cellular structures | Fluorescence Lifetime Microscopy
Elucidating cellular structures | Structured Illumination techniques
Elucidating cellular structures | Superresolution Microscopy
Elucidating cellular structures | Structure determination of macromolecular complexes by cryo-electron microscopy in vitro and in situ
Visualizing sub-cellular organization using soft x-ray tomography
Elucidating Cellular Structures | Atomic Force Microscopy
Superresolution Near-field Optical Microscopy
Elucidating cellular structures | CARS Microscopy
Elucidating cellular dynamics: Quantitative Fluorescent Speckle Microscopy
Elucidating cellular dynamics | FRAP
Elucidating cellular dynamics | Fluorescence Correlation Spectroscopy
Elucidating cellular dynamics | Image Correlation Spectroscopy
Elucidating cellular dynamics | Single molecule tracking
Protein Folding | Combining simulation and experiment to map protein folding (overview)
Energetics of Protein Folding
Globular Proteins | Fast events in protein folding
Globular Proteins | Intermediates in protein folding
Globular Proteins | Characterization of the denatured state
Globular Proteins | Single Molecule Spectroscopy in Protein Folding
Force-induced unfolding
Protein and Nucleic Acid Folding | Domain swapping in Proteins
Intrinsically Disordered Proteins
Globular Proteins | Chaperones and protein folding
Globular Proteins | Protein switches
The folding of repeat proteins
The membrane factor: biophysical studies of alpha helical transmembrane protein folding
Nucleic Acid Folding | Prediction of nucleic acid structure
Nucleic Acid Folding | Effect of protein binding on RNA folding
General Theoretical Considerations
Actin | Structure and Dynamic States of Actin Filaments
Actin | Actin Filament Nucleation and Elongation
Mechanical Properties of Actin Networks
Microtubules | Structure of Microtubules
Microtubules | Microtubule Nucleation, Polymerization and Mechanics
Microtubules | Force Generation By Dynamic Microtubule Polymers
Myosin | Myosin Motors: Structural Aspects and Functionality
Myosin | Kinetics of Myosin
Myosin | Single Molecule Fluorescence Techniques for Myosin
Myosin | Single Molecule Force Measurements on Myosins
Muscle | Muscle Fiber Mechanics and Force Generation
Muscle | Muscle Spectroscopy
Muscle | Thin Filament Regulation
Muscle | Smooth Muscle and Myosin Regulation
Intracellular transport: relating single-molecule properties to in vivo function
Non-Muscle Motility | Mitosis
Kinesin | Kinesin Structure & Biochemistry
Kinesin | Single-molecule Mechanics
Kinesin | Kinesin-related proteins: one engine, many machines
Dynein | Cytoplasmic Dynein: its ATPase Cycle and ATPase-dependent Structural Changes
Dynein | The Mechanics of Dynein Motility
Dynein | Axonemal Motility
Nucleic Acid Motors | DNA processing enzymes
Nucleic Acid Motors | The Ribosome
Nucleic Acid Motors | Viral Portal Motors
Lipid Bilayers | Lipid Bilayer Structure
Lipid Bilayers | Conformational Dynamics of Lipids in Membranes
Lipid Bilayers | Membrane phases and domains
Lipid Bilayers | Atomic Force Microscopy and Fluorescence Microscopy
Membrane Proteins | Membrane Protein Structures
Membrane Proteins | Solution NMR of membrane Proteins
Membrane Proteins | AFM and EM of Membrane Proteins
Membrane Proteins | Structure and Folding of Outer Membrane Proteins
Protein Interactions with Membranes | Interactions of Antimicrobial Peptides with Lipid Bialyers
Protein Interactions with Membranes | Membrane Electrostatics
Protein Interactions with Membranes | Pore Forming Toxins
Protein Interactions with Membranes | Membrane Recruitment of Signaling Domains
Protein Interactions with Membranes | Supported Membranes - Structure and Interactions
Protein Interactions with Membranes | Membrane Protein-Lipid Match and Mismatch
Membrane Conformational Transitions | Membrane Fusion - general overview
Membrane Conformational Transitions | Mechanisms of Enveloped Virus Entry by Membrane Fusion
Membrane Dynamics | Single Molecule Tracking in Membranes
Membrane Dynamics | Computational Methods to Model Membrane Dynamics
Detergent interactions with lipid bilayers
Molecular Modeling approaches to understand mechanisms in Voltage-Gated Channels
Connexin Channels
Structure-function correlates of Glutamate-Gated ion channels
Gating Dynamics of the Potassium Channel Pore
Biophysics of TRP channels
Structure and mechanisms in chloride channels
Biophysics of Ceramide Channels
Voltage gated proton channels
Store-operated Calcium Channels
Bacterial Toxin channels
Viral channels
Structure-Function Correlates in Plant Ion Channels
Biophysics of cell-matrix adhesion
Biophysics of selectin-mediated cell-cell adhesions
Biophysics of cadherin-mediated cell-cell adhesion
Biophysics of cell division - Understanding how dividing cells change shape
Biophysics of bacterial cell growth and division
Biophysics of three-dimensional cell motility
Cell microrheology
Biophysics of nuclear organization and dynamics
Cell-extracellular matrix mechanobiology in cancer
Biophysics of cell motility
Biophysics of cell developmental processes: A lasercutter’s perspective
Biophysics of bacterial organization
Structure and function of ATP synthase
Structure-function relationships in P-type ATPases
Rotational catalysis by the ATP synthase
The Rotary Bacterial Flagellar Motor
Electron Transfer Chains: Structures, Mechanisms and Energy Coupling
Light capture in photosynthesis
Photosystems of bacteria and plants
A B C Transporters
The mitochondrial family of transport proteins
Bacteriorhodopsin and Related Proteins
Transporters and Co-Transporters in Theory and Practice
Structure function relationships in membrane transport prtotreins of the MFS and leu-T families (approximate)
In silico approches to structure and function of cell components and their aggregates | Coarse grained methods: Theory
In silico approches to structure and function of cell components and their aggregates | Coarse grained methods:Applications to allosteric proteins
In silico approches to structure and function of cell components and their aggregates | Coarse grained methods: Applications to membranes
In silico approches to structure and function of cell components and their aggregates | Dynamics of very large systems: the ribosome
In silico approches to structure and function of cell components and their aggregates | New Technologies for Molecular Dynamics Simulations
Simulations of molecular machines | Transporters
Simulations of molecular machines | GPCRs
Simulations of molecular machines | Enzymes: method advances
MNDO-PSDCI Theory and the Excited States of Biological Chromophores
In silico approches to structure and function of cell components and their assemblies | Electrostatic properties
Modeling of interaction networks in the cell | Theory and mathematical methods
Mathematical modeling of complex biological systems | From genes and molecules to organs and organisms: Heart
Mathematical modeling of complex biological systems | From genes and molecules to organs and organisms: Kidney
Mathematical modeling of complex biological systems | From genes and molecules to organs and organisms: Immune System