Colloidal Gold, 2

Principles, Methods, and Applications

  • M. Hayat, Kean College of New Jersey, Union, U.S.A.

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Researchers, technicians, teachers, and students in cell biology, immunocytochemistry, histochemistry, and electron microscopy.


Book information

  • Published: October 1989
  • ISBN: 978-0-12-333928-7


"The chapters in these volumes are a measure of a healthy field of research and its applications."

"If you need to localise something within a cell, the colloidal gold series can probably tell you how to go about it."
"This treatise is timely and extremely helpful for the scientist interested in applying the colloidal gold methodology for a particular research problem... The present volumes elegantly cover broad research applications that reach beyond the traditional field of microscopy in cell biology and extend into the field of molecular biology... An attempt has been made to include a distinguished group of experts who are the originators of the techniques; many continue to play an active role in the technical development. The authors are from diverse fields, so that these books will be attractive to a wide audience including cell biologists, pathologists, virologists, and developmental and molecular biologists. The printing of the hardbound books is of prime quality, with excellent reproductions of the well-chosen micrographs... The text, printed on glossy paper, is extremely easy to read; diagrams, drawings, and tables throughout the two volumes are particularly pertinent in illustrating the principles and steps of many of the procedures. Although each chapter is written by a different author, all consistently follow a well organized pattern. The chapters begin with an introduction and historical background, then follow with preparation of probes and specimens, principles and descriptions of the methods for labeling, descriptions of the experimental results with a review of the literature, conclusions, and a list of citations to many original references. A particularly valuable part of each chapter is the assessment of the advantages, limitation, and pitfalls of a given methodology... These two volumes are a mix of reference book, student textbook, and cookbook. On the whole, the descriptions of methods are thorough and stimulating, and will be most useful to the experienced reader seeking to select the procedure most suited to a particular research problem. The in-depth account of the methodology and the extensive references provided will be of great help to those inte

Table of Contents

P. Baudhuin, P. Van der Smissen, S. Beauvois, and P.J. Courtoy, Molecular Interactions between Colloidal Gold, Proteins, and Living Cells. A.D. Hyatt, Protein A*b1Gold: Nonspecific Binding and Cross-Contamination. G.R. Newman and J.A. Hobot, Role of Tissue Processing in Colloidal Gold Methods. G.R. Newman, LR White Embedding Medium for Colloidal Gold Methods. J.A. Hobot, Lowicryls and Low Temperature Embedding for Colloidal Gold Methods. M. Bendayan, The Enzyme*b1Gold Cytochemical Approach: A Review. D.A. Handley and C.M. Arbeeny, Preparation and Application of Lipoprotein*b1Gold Complex. S. Villaschi, Preparation and Application of Albumin-Gold Complex. F.W.K. Kan and P. Pinto da Silva, Label-Fracture Cytochemistry. D. Men,try and A.I. Basbaum, Colloidal Gold Conjugates for Retrograde Neuronal Tracing. J.A. Traas, Colloidal Gold Labeling of Microtubules in Cleaved Whole Mounts of Cells. J.E. Beesley, Colloidal Gold: Immunonegative Staining Method. S.M. Pietschmann, E.H.S. Hausmann, and H.R. Gelderblom, Immunogold Labeling of Viruses In Situ. P. Buma, Study of Exocytosis with Colloidal Gold and Other Methods. E.M. Herman, Colloidal Gold Labeling of Acrylic Resin-Embedded Plant Tissues. C. Ferrari, G. De Panfilis, and G.C. Manara, Preembedding Immunogold Staining of Cell Surface-Associated Antigens Performed on Suspended Cells and Tissue Sections. K. Takata, ColloidalGold*b1Lectin*b1Ruthenium Red Method for High Voltage Electron Microscopy. H. Mar and T.N. Wight, Correlative Light and Electron Microscopic Immunocytochemistry in Pre-embedded Resin Sections with Colloidal Gold. R.A. Wolber and T.F. Beals, Streptavidin-Gold Labeling for Ultrastructural In-Situ Nucleic Acid Hybridization. R. Rohringer, Detection of Proteins with Colloidal Gold. J.F. Hainfeld, Undecagold*b1Antibody Method. M.D. Waele, Silver-Enhanced Colloidal Gold for the Detection of Leukocyte Cell Surface Antigens in Darkfield and Epipolarization Microscopy. Each chapter includes references. Index.